Cytomegalovirus DNA detection on Guthrie cards in patients with neonatal cholestasis
Cytomegalovirus DNA detection on Guthrie cards in patients with neonatal cholestasis
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DOI：
10.1136/fn.80.2.f130
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发表时间：
1999-03-01
影响因子：
4.4
通讯作者：
Lewensohn-Fuchs, I
中科院分区：
文献类型：
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作者：
Fischler, B;Rodensjö, P;Lewensohn-Fuchs, I
Aim-To时间巨细胞病毒（CMV）感染的患者（n=39）与CMV相关的新生儿胆汁淤积通过分析CMV DNA的古特里卡采样在3 day. Methods-CMV感染诊断血清学/尿液分离或CMV DNA检测（聚合酶链反应）在肝活检标本。为了确定感染时间，从储存的古特里卡片中冲压出干血滤纸片。酚-氯仿提取后，CMV DNA检测巢式polymerasechainreaction.Results-All卡从控制儿童（n=8）先天性巨细胞病毒检测阳性，没有阴性对照（n=4）这样做。39例胆汁淤积性婴儿中有2例CMV DNA阳性，其母亲在妊娠后半期出现与感染相符的血清学体征。所有其他胆汁淤积的婴儿检测negative.Conclusions-CMV DNA在大多数使用古特里卡的儿童中未检测到，这表明感染发生在出生时或出生后不久。
Aim-To time the onset of cytomegalovirus (CMV) infection in patients (n=39) with CMV associated neonatal cholestasis by analysing CMV DNA on Guthrie cards sampled at 3 days of age.Methods-CMV infection was diagnosed by serology/urine isolation or by CMV DNA detection (polymerase chain reaction) in liver biopsy specimens. In order to time the infection dry blood filter paper discs were punched out from stored Guthrie cards. After phenol-chloroform extraction CMV DNA was detected by nested polymerase chain reaction.Results-All cards from control children (n=8) with congenital CMV tested positive; none of the negative controls (n=4) did so. Two of 39 cholestatic infants were CMV DNA positive; their mothers had serological signs compatible with infection during the second half of the pregnancy. All other cholestatic infants tested negative.Conclusions-CMV DNA was not detected in most of the children using Guthrie cards, suggesting that infection developed at or soon after birth.