Pallidifloside D, a saponin glycoside constituent from Smilax riparia, resist to hyperuricemia based on URAT1 and GLUT9 in hyperuricemic mice
Pallidifloside D, a saponin glycoside constituent from Smilax riparia, resist to hyperuricemia based on URAT1 and GLUT9 in hyperuricemic mice
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Pallidifloside D（一种来自水茯苓的皂苷成分）基于 URAT1 和 GLUT9 在高尿酸血症小鼠中抵抗高尿酸血症
DOI：
10.1016/j.jep.2014.09.034
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发表时间：
2014-11-18
影响因子：
5.4
通讯作者：
Zhang, Yan-Wen
中科院分区：
文献类型：
--
作者：
Wu, Xiao-Hui;Ruan, Jin-Lan;Zhang, Yan-Wen
民族药理学相关性：牛尾菜（Smilax riparia，SR）的根和根茎被称为“牛尾菜”，被认为是治疗高尿酸血症和痛风症状的有效成分。本研究旨在从川楝子总皂苷中分离出一种名为pallidifloside D的皂苷苷，并在氧嗪酸钾诱导的高尿酸血症小鼠模型中检测其降低血清尿酸水平的作用。材料和方法：我们检测了5、10和20 mg/kg剂量的苍白球苷D对血清尿酸水平（S-UA）的影响，高尿酸血症小鼠的血清肌酐（S-Cr）和血尿素氮（BUN）水平。采用比色法检测pallidifloside D对高尿酸血症小鼠XOD活性的影响，并采用Western Blotting法检测pallidifloside D对高尿酸血症小鼠mURAT 1、mGLUT 9和mOTA 1蛋白水平的影响。5，10和20 mg/kg（分别为p < 0.05、p < 0.01和p < 0.01）。Pallidifloside D可剂量依赖性下调高尿酸血症小鼠肾脏mURAT 1蛋白表达水平（分别为p < 0.05、p < 0.01和p < 0.001），mGLUT 9蛋白表达呈剂量依赖性下调（分别为p < 0.05和p < 0.01）。这些结果表明，pallidifloside D通过降低肾脏mURAT 1和GLUT 9，其有助于增强尿酸排泄并减轻高尿酸血症诱导的肾功能障碍。(C)2014爱思唯尔爱尔兰有限公司版权所有。
Ethnopharmacological relevance: The roots and rhizomes of Smilax riparia (SR), called "Niu-Wei-Cai" in traditional Chinese medicine (TCM), are believed to be effective in treating hyperuricemia and gout symptoms. This study was designed to isolate a saponin glycoside named pallidifloside D from the total saponins of Smilax riparia and to examine its effect in reducing serum uric acid levels in a hyperuricemic mouse model induced by potassium oxonate.Materials and methods: We examined the effects of pallidifloside D treated with 5, 10 and 20 mg/kg on serum uric acid levels (S-UA), Serum creatinine (S-Cr) and blood urea nitrogen (BUN) levels in a hyperuricemic mouse. A colorimetric method was used to evaluate the effects of pallidifloside D on the XOD activities, and Western Blotting analysis were carried out to observe protein levels of mURAT1, mGLUT9 and mOTA1 in hyperuricemic mice after treatment with pallidifloside D.Results: The levels of serum uric acid levels (SuA) were suppressed significantly with dose-dependence by pallidifloside D treated with 5, 10 and 20 mg/kg (p < 0.05, p < 0.01 and p < 0.01 respectively). Pallidifloside D could down-regulate the expression levels of renal mURAT1 protein in hyperuricemic mice in a dose-dependent manner (p < 0.05, p < 0.01, and p < 0.001 respectively), and the protein levels of mGLUT9 could be down-regulated with dose-dependence (p < 0.05 and p < 0.01 respectively) by pallidifloside D at the dose of 10 and 20 mg/kg.Conclusion: These results suggest that pallidifloside D possesses a potent uricosuric effect in hyperuricemic mice through decreasing renal mURAT1 and GLUT9, which contribute to the enhancement of uric acid excretion and attenuate hyperuricemia-induced renal dysfunction. (C) 2014 Elsevier Ireland Ltd. All rights reserved.