Transgenic Xenopus laevis strain expressing Cre recombinase in muscle cells
Transgenic Xenopus laevis strain expressing Cre recombinase in muscle cells
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DOI：
10.1002/dvdy.20880
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发表时间：
2006-08-01
影响因子：
2.5
通讯作者：
Ryffel, Gerhart U.
中科院分区：
文献类型：
--
作者：
Waldner, Christoph;Sakamaki, Kazuhiro;Ryffel, Gerhart U.
对于非洲爪蟾基因功能的可重复分析，使用转基因菌株是一种很有前途的方法，但在调查干扰发育的因素时存在局限性。因此，诱导系统是有吸引力的替代方案，并且基于重组酶的二元系统是最通用的方法。我们以前已经表明，Cre和FLP重组酶在非洲爪蟾中是活跃的，可以诱导相应的报告菌株中的沉默报告基因。在这里，我们描述了建立转基因非洲爪蟾菌株A7表达Cre重组酶的肌肉特异性心脏肌动蛋白启动子的控制下。在与几种不同的报告菌株杂交后，A7能够以肌肉特异性方式诱导EYFP、DsRed2或LacZ报告基因。这第一个Cre表达菌株允许有条件激活肌肉细胞中的任何感兴趣的基因，因此，开辟了使用重组酶作为一种新的实验策略在非洲爪蟾。
For reproducible analyses of gene function in Xenopus, the use of transgenic strains is a promising approach but has limitations when investigating factors interfering with development. Therefore, inducible systems are attractive alternatives, and a binary system based on recombinases is a most versatile approach. We have shown previously that Cre and FLP recombinases are active in Xenopus laevis and can induce a silent reporter gene in a corresponding reporter strain. Here, we describe the establishment of the transgenic Xenopus laevis strain A7 expressing Cre recombinase under the control of the muscle-specific cardiac actin promoter. Upon crossing to several distinct reporter strains, A7 is able to induce EYFP, DsRed2, or LacZ reporter genes in a muscle-specific manner. This first Cre-expressing strain allows conditional activation of any gene of interest in muscle cells and, thus, opens up the use of recombinases as a new experimental strategy in Xenopus.