MOLECULAR-CLONING OF THE GENE ENCODING THE PUTATIVE POLYMERASE OF MOUSE HEPATITIS CORONAVIRUS, STRAIN A59
MOLECULAR-CLONING OF THE GENE ENCODING THE PUTATIVE POLYMERASE OF MOUSE HEPATITIS CORONAVIRUS, STRAIN A59
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DOI：
10.1016/0042-6822(89)90520-5
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发表时间：
1989-07-01
期刊：
影响因子：
3.7
通讯作者：
WEISS, SR
中科院分区：
文献类型：
--
作者：
PACHUK, CJ;BREDENBEEK, PJ;WEISS, SR
构建了代表冠状病毒小鼠肝炎病毒A59株（MHV-A59）基因组RNA的互补DNA（cDNA）文库。从这些文库中选择克隆以形成跨越整个基因A（推定的病毒聚合酶基因）的线性图谱。该基因的长度约为23 kb，比以前的估计大得多。对基因组5“末端区域的序列分析表明存在所有mRNA上发现的66个核苷酸的前导序列。 5“末端区域的二级结构分析表明，前导序列的转录终止于核苷酸66的区域。前2000个核苷酸的序列与报道的密切相关的MHV JHM株非常相似，并且可能编码p28，p28是一种碱性蛋白，尽管是病毒聚合酶的组分（L.索埃角K. Shieh，S.贝克，M。F. Chang和M. M. C. Lai，1987，J. Virol.，61，3968-3976）。基因A包含两个在基因间区域发现的共有序列。一个与5“前导序列相邻，另一个位于基因B翻译起始密码子的上游。
Complementary DNA (cDNA) libraries were constructed representing the genome RNA of the coronavirus mouse hepatitis virus, strain A59 (MHV-A59). From these libraries clones were selected to form a linear map across the entire gene A, the putative viral polymerase gene. This gene is approximately 23 kb in length, considerably larger than earlier estimates. Sequence analysis of the 5'' terminal region of the genome indicates the presence of the 66-nucleotide leader that is found on all mRNAs. Secondary structure analysis of the 5'' terminal region suggests that transcription of leader terminates in the region of nucleotide 66. The sequence of the first 2000 nucleotides is very similar to that reported for the closely related JHM strain of MHV and potentially encodes p28, a basic protein though to be a component of the viral polymerase (L. Soe, C. K. Shieh, S. Baker, M. F. Chang, and M. M. C. Lai, 1987, J. Virol., 61, 3968-3976). Gene A contains two of the consensus sequences found in intergenic regions. One is adjacent to the 5'' leader sequence and the other is upstream from the initiation codon for translation of gene B.