Resolution of polycistronic RNA by SL2 trans-splicing is a widely conserved nematode trait.
Resolution of polycistronic RNA by SL2 trans-splicing is a widely conserved nematode trait.
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DOI：
10.1261/rna.076414.120
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发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
Connolly B
中科院分区：
文献类型：
--
作者：
Wenzel M;Johnston C;Müller B;Pettitt J;Connolly B
剪接前导序列的反式剪接对于真核操纵子产生的多顺反子RNA的加工和翻译至关重要。In C. elegans是一个专门的剪接前导序列，SL 2为来自多顺反子RNA的未加帽前体mRNA提供5′端。对其他线虫的研究表明，SL 2型反式剪接是一个相对较新的创新，仅限于杆形线虫，含有C。Elegans及其近亲。在这里，我们进行了调查的转录组范围内剪接前导反式剪接旋毛虫，一个远亲的C。具有15个剪接前导序列的特别多样的库。通过系统地比较每个剪接前导序列的反式剪接事件的基因组背景，我们鉴定了T. spiralis剪接的前导序列，专门用于加工多顺反子RNA--这些T。spiralis剪接前导RNA具有一个完全保守的茎环基序，以前显示是必需的SL 2型反式剪接在C。优美的我们发现，这些SL 2型剪接领导人的基因反式剪接组织在操纵子的方式，短顺反子间的距离。T.的一个子集。spiralis操纵子与C.线虫操纵子我们的工作实质上修改了我们的理解，线虫剪接前导反式剪接，表明SL 2反式剪接是一个主要的机制，线虫多顺反子RNA加工，这可能已经演变之前的辐射的线虫。这项工作具有重要的意义，在线虫和其他真核生物的基因组注释管道与操纵子基因组织的改进。
Spliced leader trans-splicing is essential for the processing and translation of polycistronic RNAs generated by eukaryotic operons. In C. elegans, a specialized spliced leader, SL2, provides the 5′ end for uncapped pre-mRNAs derived from polycistronic RNAs. Studies of other nematodes suggested that SL2-type trans-splicing is a relatively recent innovation, confined to Rhabditina, the clade containing C. elegans and its close relatives. Here we conduct a survey of transcriptome-wide spliced leader trans-splicing in Trichinella spiralis, a distant relative of C. elegans with a particularly diverse repertoire of 15 spliced leaders. By systematically comparing the genomic context of trans-splicing events for each spliced leader, we identified a subset of T. spiralis spliced leaders that are specifically used to process polycistronic RNAs—the first examples of SL2-type spliced leaders outside of Rhabditina. These T. spiralis spliced leader RNAs possess a perfectly conserved stem–loop motif previously shown to be essential for SL2-type trans-splicing in C. elegans. We show that genes trans-spliced to these SL2-type spliced leaders are organized in operonic fashion, with short intercistronic distances. A subset of T. spiralis operons show conservation of synteny with C. elegans operons. Our work substantially revises our understanding of nematode spliced leader trans-splicing, showing that SL2 trans-splicing is a major mechanism for nematode polycistronic RNA processing, which may have evolved prior to the radiation of the Nematoda. This work has important implications for the improvement of genome annotation pipelines in nematodes and other eukaryotes with operonic gene organization.
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影响因子：
10.7
作者：
Danks, Gemma B.;Raasholm, Martina;Thompson, Eric M.
通讯作者：
Thompson, Eric M.
DOI：
10.1895/wormbook.1.5.2
发表时间：
2012-11-20
期刊：
WormBook : the online review of C. elegans biology
影响因子：
--
作者：
Blumenthal, Thomas
通讯作者：
Blumenthal, Thomas
影响因子：
10.5
作者：
Evans, D;Perez, I;Blumenthal, T
通讯作者：
Blumenthal, T
DOI：
10.1093/bioinformatics/bts565
发表时间：
2012-12-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Fu L;Niu B;Zhu Z;Wu S;Li W
通讯作者：
Li W
影响因子：
4.5
作者：
Guiliano DB;Blaxter ML
通讯作者：
Blaxter ML