Effects of bagging on fruit flavor quality and related gene expression of AsA synthesis in Actinidia eriantha
Effects of bagging on fruit flavor quality and related gene expression of AsA synthesis in Actinidia eriantha
复制标题
套袋对毛花猕猴桃果实风味品质及AsA合成相关基因表达的影响
DOI：
10.1016/j.scienta.2019.05.038
复制
发表时间：
2019-10-15
影响因子：
4.3
通讯作者：
Xu Xiaobiao
中科院分区：
文献类型：
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作者：
Liao Guanglian;He Yanqun;Xu Xiaobiao
选取3个高抗坏血酸（AsA）含量的毛花猕猴桃株系，研究套袋和不套袋（对照）对果实外观和内在品质以及参与抗坏血酸合成的关键基因转录水平的影响。与对照相比，套袋和对照在可溶性固形物、干物质、可溶性糖和可滴定酸方面无显著差异，但套袋显著改善果实外观和果肉颜色，降低叶绿素、类胡萝卜素和AsA含量。参与AsA合成的关键基因表达情况显示，3个株系中套袋的GME、GMP、GGP、GPP和GaIDH表达与对照不一致，而GaILDH、GalUR和DHAR表达趋势相同且显著低于对照。因此，推测GaILDH、GalUR和DHAR的不同表达是套袋后AsA降低的关键因素。毛花猕猴桃套袋可有效改善果实外观和果肉颜色，提高果实观赏价值，对果实糖酸含量影响较小。虽然AsA含量降低，但AsA含量（>1250mg/100g）仍处于较高水平。
Three lines of Actinidia eriantha with high AsA content were used to determine die effects of bagging and control (never bagged) on fruit external and internal qualities, as well as transcript levels for key genes involved in ascorbic acid (AsA) synthesis. Compared with the control, there was no significant difference between bagging and control in soluble solid, dry matter, soluble sugar and titrable acid, but bagging would significantly improve fruit appearance and flesh color, reduced the content of chlorophyll, carotenoid and AsA. The key gene expression involved in AsA synthesis showed that the expressions of GME, GMP, GGP, GPP and GaIDH in bagging were inconsistent with the control on three lines. Whereas GaILDH, GalUR and DHAR had the same expression trend and significantly lower than the control. Therefore, we speculated that the different expression of GaILDH, GalUR and DHAR was the key factors for decrease of AsA after bagging. Bagging on A. eriantha can effectively improve the appearance and flesh color of the fruit, improve the ornamental value of the fruit, and has little effect on the sugar and acid content of the fruit. Although the AsA content was reduced, the AsA content (> 1250 mg/100 g) was still at a high level.