Serine hydroxymethyltransferase participates in the synthesis of cysteine-rich storage proteins in rice seed
Serine hydroxymethyltransferase participates in the synthesis of cysteine-rich storage proteins in rice seed
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丝氨酸羟甲基转移酶参与水稻种子中富含半胱氨酸的储存蛋白的合成
DOI：
10.1016/j.plantsci.2021.111049
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发表时间：
2021
期刊：
影响因子：
5.2
通讯作者：
Toshihiro Kumamaru
中科院分区：
文献类型：
--
作者：
Hiroaki Matsusaka;Masako Fukuda;Ammar Elakhdar;Toshihiro Kumamaru
低水平的富半胱氨酸蛋白（lcrp）突变表明富半胱氨酸（CysR）脯胺、α-球蛋白和谷蛋白的减少。为了确定导致lcrpmutation的原因，阐明其功能，并阐明CysR蛋白在蛋白体（PBs）形成中的作用，对lcrpmutant进行了分析。我们构建了theLCRPgene的连锁图谱，并对定位区域内的一个预测基因进行基因组DNA测序，结果表明lcrpenc编码一种丝氨酸羟甲基转移酶，该酶参与硫同化途径中单碳代谢的甘氨酸-丝氨酸相互转化。硫同化途径中l- ser、Gly和Met的水平显著高于野生型。由于该突变对茎和根的生长都有影响，因此研究了在培养基中添加氨基酸和其他化合物对硫同化途径的影响。观察到被核糖体附着膜包围的电子发光PBs在作物种子中积累了缺乏半胱氨酸的脯胺。此外，与WT相比，含谷蛋白的PBs更小且扭曲。这些分析表明，富含半胱氨酸的蛋白质在水稻中PBs的形成中起重要作用。
Thelow level of cysteine-rich proteins(lcrp) mutation indicates a decrease in cysteine-rich (CysR) prolamines, α-globulin, and glutelin. To identify the causing factor oflcrpmutation, to elucidate its function, and to elucidate the role of CysR proteins in the formation of protein bodies (PBs),lcrpmutant was analyzed. A linkage map of theLCRPgene was constructed and genomic DNA sequencing of a predicted gene within the mapped region demonstrated thatLCRPencodes a serine hydroxymethyltransferase, which participates in glycine-serine interconversion of one-carbon metabolism in the sulfur assimilation pathway. The levels ofl-Ser, Gly, and Met in the sulfur assimilation pathway in thelcrpseeds increased significantly compared to that in the wildtype (WT). As thelcrpmutation influences the growth of shoot and root, the effects of the addition to the medium of amino acids and other compounds on the sulfur assimilation pathway were studied. Electron-lucent PBs surrounded by ribosome-attached membranes were observed accumulating cysteine-poor prolamines in thelcrpseeds. Additionally, glutelin-containing PBs were smaller and distorted in thelcrpseeds compared to those in the WT. These analyses of PBs in thelcrpseeds suggest that cysteine-rich proteins play an important role in the formation of PBs in rice.