HAMSTER PREPROGLUCAGON CONTAINS THE SEQUENCE OF GLUCAGON AND 2 RELATED PEPTIDES
HAMSTER PREPROGLUCAGON CONTAINS THE SEQUENCE OF GLUCAGON AND 2 RELATED PEPTIDES
复制标题
DOI：
10.1038/302716a0
复制
发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
MULLENBACH, GT
中科院分区：
文献类型：
--
作者：
BELL, GI;SANTERRE, RF;MULLENBACH, GT
胰高血糖素是由内分泌胰腺的A细胞合成的29个氨基酸的多肽激素1 -4。它的主要作用部位是肝脏，刺激糖原分解、糖异生和生酮。在哺乳动物中，生物合成研究表明胰高血糖素来源于分子量（Mr）约为18，000的前体，其比胰高血糖素大5至6倍。还描述了来自胃、肠、脑和唾液腺的各种大小的含胰高血糖素多肽和免疫反应物3。在这里，我们已经确定了仓鼠胰腺前胰高血糖素原的结构从其cDNA序列。这种180个氨基酸的前体含有胰高血糖素序列和串联排列的两个胰高血糖素样多肽。前体还含有几种非胰高血糖素多肽的序列，这表明在哺乳动物中，胰高血糖素和非胰高血糖素以及含高血糖素多肽都可以通过组织特异性加工从共同的前体衍生。我们已经初步确定了每一种胰高血糖素样免疫反应物，这些免疫反应物已经被描述为胰高血糖素原的序列，并提出了一个处理胰腺胰高血糖素原的方案。
Glucagon is a 29-amino acid polypeptide hormone synthesized by the A cells of the endocrine pancreas1–4. Its primary site of action is the liver where it stimulates glycogenolysis, gluconeogenesis and ketogenesis. In mammals, biosynthetic studies have shown that glucagon is derived from a precursor of molecular weight (Mr) approximately 18,000 which is five to six times larger than glucagon5. Glucagon-containing polypeptides and immunoreactants of various sizes have also been described from stomach, intestine, brain and salivary gland3. Here, we have determined the structure of hamster pancreatic preproglucagon from the sequence of its cDNA. This 180-amino acid precursor contains the sequence of glucagon and two glucagon-like polypeptides arranged in tandem. The precursor also contains the sequences of several non-pancreatic glucagon-containing polypeptides which suggests that, in mammals, both pancreatic and non-pancreatic glucagon and glucagon-containing polypeptides may be derived from a common precursor by tissue-specific processing. We have tentatively identified each of the glucagon-like immunoreactants which have been described with respect to the sequence of proglucagon and have proposed a scheme for the processing of pancreatic proglucagon.