Cell proliferation and neurogenesis in the adult telencephalon of the newt, Cynops pyrrhogaster
Cell proliferation and neurogenesis in the adult telencephalon of the newt, Cynops pyrrhogaster
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蝾螈成年端脑的细胞增殖和神经发生
DOI：
10.1111/dgd.12826
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
I.
中科院分区：
文献类型：
--
作者：
Iwasa;A.;Hanaoka;N.;Ohwada;K.;Iwamuro;S.;Toyoda;F.;Kikuyama;S.;Hasunuma;I.
有尾目两栖动物有再生几个器官的能力，包括大脑。由于这个原因，在这些物种的大脑的某些部分消融后的神经发生的研究取得了显着进展。然而，关于增殖细胞的特征和命运以及正常条件下新产生的神经元的功能的详细信息仍然有限。在这项研究中，我们专注于研究日本红腹蝾螈成年大脑中的增殖区和神经发生区以及增殖细胞的命运，以阐明成年期神经发生的意义。我们发现端脑侧脑室近端区和间脑视前区是成人脑细胞持续增殖的主要部位。此外，我们通过5-乙炔基-2 ′-脱氧尿苷（EdU）标记和免疫组织化学结合使用干细胞标记物Sox 2和神经元标记物NeuN的抗体来表征增殖细胞并分析神经发生。EdU注射后24小时，大多数EdU阳性细胞为Sox 2免疫阳性，而EdU阳性信号和NeuN免疫反应性未共定位。EdU注射后2个月，EdU阳性信号与Sox 2免疫反应性的共定位率降至约10%，而EdU阳性信号与NeuN免疫反应性的共定位率增至约60%。此外，一部分EdU掺入的细胞发育成γ-氨基丁酸产生细胞，其被认为具有中间神经元的功能。在此基础上，讨论了新生神经元的意义，特别是它们的繁殖行为。
Urodele amphibians have the ability to regenerate several organs, including the brain. For this reason, the research on neurogenesis in these species after ablation of some parts of the brain has markedly progressed. However, detailed information on the characteristics and fate of proliferated cells as well as the function of newly generated neurons under normal conditions is still limited. In this study, we focused on investigating the proliferative and neurogenic zones as well as the fate of proliferated cells in the adult brain of the Japanese red‐bellied newt to clarify the significance of neurogenesis in adulthood. We found that the proximal region of the lateral ventricles in the telencephalon and the preoptic area in the diencephalon were the main sites for continuous cell proliferation in the adult brain. Furthermore, we characterized proliferative cells and analyzed neurogenesis through a combination of 5‐ethynyl‐2′‐deoxyuridine (EdU) labeling and immunohistochemistry using antibodies against the stem cell marker Sox2 and neuronal marker NeuN. Twenty‐four hours after EdU injection, most of the EdU‐positive cells were Sox2‐immunopositive, whereas, EdU‐positive signals and NeuN‐immunoreactivities were not colocalized. Two months after EdU injection, the colocalization ratio of EdU‐positive signals with Sox2‐immunoreactivities decreased to approximately 10%, whereas the ratio of colocalization of EdU‐positive signals with NeuN‐immunoreactivities increased to approximately 60%. Furthermore, a portion of the EdU‐incorporated cells developed into γ‐aminobutyric acid‐producing cells, which are assumed to function as interneurons. On the basis of these results, the significance of newly generated neurons was discussed with special reference to their reproductive behavior.