Effect of polyphenol-containing azuki bean (vigna angularis) extract on blood pressure elevation and macrophage infiltration in the heart and kidney of spontaneously hypertensive rats
Effect of polyphenol-containing azuki bean (vigna angularis) extract on blood pressure elevation and macrophage infiltration in the heart and kidney of spontaneously hypertensive rats
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DOI：
10.1111/j.1440-1681.2007.04743.x
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发表时间：
2008-01-01
影响因子：
2.9
通讯作者：
Sagai, Masaru
中科院分区：
文献类型：
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作者：
Sato, Shin;Mukai, Yuuka;Sagai, Masaru
1.高血压是心肌梗死和肾损害的主要危险因素，并且还显示其具有增加活性氧形成的促炎作用。巨噬细胞浸润已被认为在高血压的发病机制中发挥作用。红豆中含有原花青素，这是一组多酚类化合物，具有显著的体外自由基清除活性。因此，本研究的目的是探讨含多酚的红豆提取物（ABE）对自发性高血压大鼠（SHR）收缩压（SBP）和心脏、肾脏巨噬细胞浸润的影响.将自发性高血压大鼠和对照血压正常的Wistar-Kyoto（WKY）大鼠分为两组，分别饲喂0和0.8%的ABE。检测尾动脉收缩压（SBP）和心脏、肾脏巨噬细胞动力学.在整个治疗期间，SHR组的SBP高于年龄匹配的WKY大鼠。治疗8周后，ABE治疗组SHR的SBP升高幅度明显小于未治疗组.在SHR和WKY大鼠的肾脏和心脏中，与不存在NADH或NADPH的水平相比，烟酰胺腺嘌呤二核苷酸（NADH）或烟酰胺腺嘌呤二核苷酸磷酸（NADPH）刺激的超氧化物（）产生增加。未处理SHR的肾脏中NADPH刺激的水平显著高于未处理WKY大鼠。ABE处理组SHR的血清中TNF-α水平较未处理组显著降低.在免疫组化分析中，在心脏和肾脏的肾小球和肾小管中的巨噬细胞的数量显着高于ABE未处理的SHR比ABE未处理的WKY大鼠。相反，与未处理的SHR相比，ABE处理的SHR中巨噬细胞的数量显著减少。WKY大鼠和SHR的收缩压与心脏、肾小管间质和肾小球中ED 1阳性巨噬细胞数量呈显著正相关.总之，本研究的结果表明，ABE减弱SBP和巨噬细胞浸润的心脏，以及在肾小球和肾小管，在我们的SHR模型的升高。
1. Hypertension is a major risk factor for myocardial infarction and renal damage, and it has also been shown to have proinflammatory actions that increase the formation of reactive oxygen species. Macrophage infiltration has been suggested to play a role in the pathogenesis of hypertension. Azuki beans are known to contain pro-anthocyanidins, a group of polyphenolic bioflavonoids with remarkable radical-scavenging activities in vitro. Therefore, the aim of the present study was to investigate the effect of polyphenol-containing azuki bean extract (ABE) on systolic blood pressure (SBP) and macrophage infiltration in the heart and kidney of spontaneously hypertensive rats (SHR).2. Spontaneously hypertensive rats and control normotensive Wistar-Kyoto (WKY) rats were divided into two groups fed either 0 or 0.8% ABE in their diets. Tail SBP and macrophage kinetics in the heart and kidney were examined.3. The SBP of the SHR group was higher than that of age-matched WKY rats throughout the treatment period. After 8 weeks of treatment, the increased SBP in ABE-treated SHR was significantly less than that in untreated SHR.4. Nicotinamide adenine dinucleotide ( NADH) or nicotinamide adenine dinucleotide phosphate (NADPH)-stimulated superoxide () production was enhanced in the kidney and heart in SHR and WKY rats compared with levels in the absence of NADH or NADPH. The NADPH-stimulated levels in the kidney in untreated SHR was significantly higher than that in untreated WKY rats. The levels in ABE-treated SHR were significantly decreased compared with the untreated SHR group.5. In immunohistochemical analyses, the number of macrophages in the heart and in the glomeruli and tubulointerstitium of the kidney was significantly higher in ABE-untreated SHR than in ABE-untreated WKY rats. Conversely, there was a significant decrease in the number of macrophages in ABE-treated SHR compared with the untreated SHR. There were significant positive correlations between SBP and the number of ED1-positve macrophages in the heart and tubulointerstitial and glomerular areas of the kidney in WKY rats and SHR.6. In conclusion, the results of the present study suggest that ABE attenuates the elevation of SBP and macrophage infiltration in the heart, as well as in the glomeruli and tubulointerstitium of the kidney, in our SHR model.