Growth, immunity, and ammonia excretion of albino and normal Apostichopus japonicus (Selenka) feeding with various experimental diets
Growth, immunity, and ammonia excretion of albino and normal Apostichopus japonicus (Selenka) feeding with various experimental diets
复制标题
不同实验饲料喂养的白化刺参和正常刺参的生长、免疫和氨排泄
DOI：
10.1111/anu.12646
复制
发表时间：
2017
影响因子：
3.5
通讯作者：
Yang Hongsheng
中科院分区：
文献类型：
--
作者：
Xia Sudong;Li Man;Zhang Libin;Rahman M. M.;Xu Qinzeng;Sun Lina;Liu Shilin;Yang Hongsheng
研究了6种饲料对白化病仿刺参和正常仿刺参生长性能、排氨能力和免疫力的影响。采用析因设计，因子为饲料类型（6个水平）和A. lovis的颜色（2个水平）。将30只随机选择的白化日本按蚊饲养在18个蓝色塑料水族箱中，每个水族箱（60 × 50 × 30 cm 3）分为6组，一式三份，正常日本按蚊使用相同的设置。每组动物喂食六种实验饮食中的一种。采用酸不溶性灰分（AIA）含量法分析表观干物质消化率（AMD）和表观粗蛋白消化率（ACPD）。实验结束时，收获所有日本曲霉并称重以计算生长参数。称重后，从每个水族箱中随机抽取6个个体用于免疫指标。结果表明，日本沼虾的所有生长参数都随着饲料中营养物质含量的降低而增加（p<0.01），而在日本沼虾的氨氮产量情况下观察到相反的结果。正常日本对虾生长良好（p<0.01），氨氮产生量低于白化日本对虾（p<0.01）。免疫力，特别是超氧化物歧化酶和溶菌酶活性，正常人比白化病人高（p<0.05）。综合考虑各指标，D1（粗蛋白、粗脂肪、碳水化合物和粗灰分含量分别为51.8、8.7、231.3、708.2g/kg）为最佳日粮。由于这项研究没有提供有关饮食中营养物质临界阈值水平的信息，因此仍需要更多的研究来优化A. patient饮食中的营养物质。在此之前，可以推荐日本对虾养殖饲料D1。
An experiment was conducted to evaluate the effects of six experimental diets on growth performance, ammonia excretion and immunity of albino and normalApostichopus japonicus. A factorial design was used, the factors being type of diets (six levels) and colour ofA. japonicus(two levels). A total of 30 randomly selected albinoA. japonicuswere housed in each (60 × 50 × 30 cm3) of 18 blue plastic aquaria to form six groups in triplicate, and the same set‐up was used for the normalA. japonicus. Each group of animals was fed with one of the six experimental diets. Apparent dry matter digestibility (ADMD) and apparent crude protein digestibility(ACPD) were analysed using acid‐insoluble ash (AIA) content method. At the end of the experiment, allA. japonicuswere harvested and weighed to calculate growth parameters. After weighing, six individuals from each aquarium were randomly sampled for immune indices. Results indicated that all growth parameters ofA. japonicusincreased with decreasing nutrient content in their diets (p< .01), whereas an opposite result was observed in case of the ammonia‐nitrogen production byA. japonicus. NormalA. japonicusgrew better (p< .01) and produced lower (p< .01) quantity of ammonia nitrogen compared to the albinoA. japonicus. Immunity particularly superoxide dismutase and lysozyme activities was higher (p< .05) in normal compared to albinoA. japonicus. Considering all measured variables, D1 (diet containing crude protein, crude lipid, carbohydrate and crude ash 51.8, 8.7, 231.3, 708.2 g/kg, respectively) was the best diet among all experimental diets. More research is still needed to optimize nutrients in the diet ofA. japonicus, as this study does not provide information about critical threshold level of nutrients in diets. Until then, diet D1 can be recommended forA. japonicusaquaculture.