X-ray diffraction studies of enkephalins. Crystal structure of [(4'-bromo) Phe4,Leu5]enkephalin.
X-ray diffraction studies of enkephalins. Crystal structure of [(4'-bromo) Phe4,Leu5]enkephalin.
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脑啡肽的 X 射线衍射研究。
DOI：
10.1042/bj2180677
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
S. Sakakibara
中科院分区：
文献类型：
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作者：
T. Ishida;M. Kenmotsu;Y. Mino;M. Inoue;T. Fujiwara;K. Tomita;T. Kimura;S. Sakakibara
为了研究[Leu5]-和[Met5]脑啡肽的构效关系，合成并结晶了[(4'-bromo)Phe4, Leu5]-、[(4'-bromo)Phe4, Met5]-和[Met5]脑啡肽。采用重原子法通过X射线衍射法测定了[(4'-溴)Phe4,Leu5]-脑啡肽的晶体结构，并通过最小二乘法精修至R=0.092。该晶体中的分子基本上具有与[Leu5]脑啡肽中发现的相同的I'型β-转角构象[Smith & Griffin (1978) Science 199, 1214-1216]。另一方面，初步的三维Patterson分析表明[(4'-bromo)Phe4,Met5]-和[Met5]脑啡肽最可能的构象都是二聚体延伸形式。基于这些见解，讨论了脑啡肽的生物活性构象与 mu 和 delta 受体的关系。
In order to investigate the structure-activity relationship of [Leu5]- and [Met5]enkephalins, [(4'-bromo)Phe4, Leu5]-, [(4'-bromo)Phe4, Met5]- and [Met5] enkephalins were synthesized and crystallized. The crystal structure of [(4'-bromo) Phe4, Leu5]- enkephalin was determined by X-ray diffraction method using the heavy atom method and refined to R = 0.092 by the least-squares method. The molecule in this crystal took essentially the same type I' beta-turn conformation found in [Leu5]enkephalin [Smith & Griffin (1978) Science 199, 1214-1216). On the other hand, the preliminary three-dimensional Patterson analyses showed that the most probable conformations of [(4'-bromo)Phe4,Met5]- and [Met5]enkephalins are both the dimeric extended forms. Based on these insights, the biologically active conformation of enkephalin was discussed in relation to the mu- and delta-receptors.