INHERENT CELLULAR DIFFERENCES MAY EXPLAIN THE DISSIMILAR SURVIVAL OF RIF-1 AND KHT TUMOR-CELLS UNDER AEROBIC AND HYPOXIC CONDITIONS
INHERENT CELLULAR DIFFERENCES MAY EXPLAIN THE DISSIMILAR SURVIVAL OF RIF-1 AND KHT TUMOR-CELLS UNDER AEROBIC AND HYPOXIC CONDITIONS
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DOI：
10.1080/09553009514550511
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发表时间：
1995-04-01
影响因子：
2.6
通讯作者：
FENTON, BM
中科院分区：
文献类型：
--
作者：
KIANI, MF;FENTON, BM
尽管先前的工作已显示 KHT 和 RIF-1 鼠肉瘤之间的放射生物学缺氧分数存在显着差异，但血管内氧合血红蛋白 (HbO(2) 饱和度）显示出较小的实质性差异。使用定量组织学技术，我们还发现 KHT 与 RIF-1 肉瘤的肿瘤细胞与最近血管之间的距离分布存在微小差异。我们在此报告这些肿瘤细胞承受以下条件的固有能力的调查结果：在有氧和缺氧条件下进行体外培养，将肿瘤分离，接种到培养皿中，并放置在气密的铝室中，并在缺氧暴露后的2.5小时内反复排空并重新填充细胞，并在标准化后使用集落存活测定法测定体外铺板效率（PE）。与有氧对照相比，在缺氧条件下暴露 16 小时 (0.4) 后，KHT 肿瘤细胞的 PE 显着低于 RIF-1 (0.6)。将缺氧暴露增加到 4.0 小时，导致 KHT 的标准化 PE 为 0.07，而 RIF-I 为 0.4。尽管这些结果支持两种肿瘤系具有不同的固有耐缺氧能力的假设，但它们并不能解释肿瘤氧合直接测量的失败。还可能涉及与放射生物学缺氧分数相关的其他因素，例如肿瘤系之间的氧扩散率或耗氧率的差异。
Although previous work has shown striking differences in radiobiological hypoxic fraction between KHT and RIF-1 murine sarcomas, intravascular oxyhaemoglobin (HbO(2) saturations have revealed less substantial variations. Using quantitative histological techniques, we have also found minor differences in the distributions of distances between tumour cells and the nearest bloods vessel for KHT versus RIF-1 sarcomas. We report here, the results of an investigation of the inherent ability of these tumour cells to withstand conditions of hypoxia by in vitro culturing under aerobic and anoxic conditions. Tumours were dissociated, seeded into culture dishes, and placed in air-tight aluminium chambers. These chambers were repeatedly evacuated and refilled with a mixture of 95% N-2 and 5% CO2 over a 2.5-h period. Following anoxic exposure, cells were removed and replated, and the in vitro plating efficiency (PE) was determined using a colony survival assay. After normalizing to aerobic controls, KHT tumour cells had a significantly lower PE, following a 16-hour exposure to anoxic conditions (0.4), than RIF-1 (0.6). Increasing the hypoxic exposure to 4.0 h resulted in normalized PEs of 0.07 for KHT versus 0.4 for RIF-I, Although these results support the hypothesis that the two tumour lines have different inherent abilities to withstand hypoxia, they do not explain the failure of direct measures of tumour oxygenation to correlate with the radiobiological hypoxic fraction. Additional factors such as differences in oxygen diffusivity or oxygen consumption rates between tumour lines may also be involved.