Molecular characterization of Carla- and potyviruses from Narcissus in China
Molecular characterization of Carla- and potyviruses from Narcissus in China
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DOI：
10.1046/j.1439-0434.2003.00674.x
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发表时间：
2003-01-01
期刊：
影响因子：
--
通讯作者：
Adams, MJ
中科院分区：
文献类型：
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作者：
Chen, J;Chen, JP;Adams, MJ
利用香石竹病毒属和马铃薯Y病毒属的保守引物，采用RT-PCR技术，从中国水仙（Narcissus tazetta var. chinensis），并进行序列测定和系统发育分析。来自福建省和上海的样品含有一种或两种马铃薯Y病毒和一种香石竹病毒。其中一个马铃薯Y病毒属（PY 1）与鸢尾严重花叶病毒、洋葱黄矮病毒和青葱黄条病毒关系较远，而另一个（仅在富江样品中，PY 2）与芜菁花叶病毒（TuMV）关系最密切。使用来自英国的温室生长的水仙进行的类似实验含有虎眼万年青花叶病毒和TuMV/PY 2簇中的另一种马铃薯Y病毒（PY 3）。与已测定的序列片段比较表明，PY 2可能是水仙黄条病毒，PY 3可能是水仙晚季黄化病毒。两个中国地点的香石竹病毒片段似乎属于同一病毒，在系统发育分析中，与马铃薯M病毒、马铃薯潜伏病毒和啤酒花潜伏病毒的亲缘关系最近。它很可能是卡拉病毒属的一个新成员，暂命名为水仙普通潜伏病毒。
Conserved carlavirus and potyvirus primers were used in reverse transcriptase-polymerase chain reaction (RT-PCR) to amplify virus fragments from Chinese narcissus (Narcissus tazetta var. chinensis ) in China and the fragments were subsequently sequenced and compared in phylogenetic analyses. Samples from Fujiang province and Shanghai contained either one or two potyviruses and a carlavirus. One potyvirus (PY1) showed a distant relationship to Iris severe mosaic virus, Onion yellow dwarf virus and Shallot yellow stripe virus, while the other (only in the Fujiang sample, PY2) was most closely related to Turnip mosaic virus (TuMV). Similar experiments using glasshouse grown Narcissus originating from the UK contained Ornithogalum mosaic virus and another potyvirus (PY3) in the TuMV/PY2 cluster. Comparisons with previously determined sequence fragments indicated that PY2 was probably Narcissus yellow stripe virus and PY3 Narcissus late season yellow virus. Carlavirus fragments from both Chinese sites seemed to be of the same virus, which was most closely related in phylogenetic analyses to Potato virus M, Aconitum latent virus and Hop latent virus. It is most probably a new member of the genus Carlavirus and has been tentatively named Narcissus common latent virus.