Effect of feeding on peripheral circadian rhythms and behaviour in mammals
Effect of feeding on peripheral circadian rhythms and behaviour in mammals
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DOI：
10.1111/j.1365-2443.2004.00769.x
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发表时间：
2004-09-01
期刊：
影响因子：
2.1
通讯作者：
Ishida, N
中科院分区：
文献类型：
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作者：
Kobayashi, H;Oishi, K;Ishida, N
虽然进食时间是哺乳动物外周组织昼夜节律的主要线索，但进食和禁食对昼夜基因表达和行为的影响尚不清楚。在这里，我们报告说，禁食不影响时钟基因，mPer 1，mPer 2和时钟控制基因，mDBP的节奏mRNA表达水平的阶段。然而，这些基因中的每一个的水平都以不同的方式发生了显着改变，并通过喂食恢复。我们还发现，喂食增强了心脏中mPer 2 mRNA表达节律的新的亮-暗周期的相移。此外，喂食比禁食更能促进行为向新的明暗周期的转变。我们的数据表明，喂养是一个重要的线索，昼夜节律的行为，以及光夹带外周时钟基因的表达。
Although feeding time is a dominant cue for circadian rhythms in mammalian peripheral tissue, the effect of feeding and fasting on circadian gene expression and behaviour is unknown. Here we report that fasting does not affect the phase of rhythmic mRNA expression levels of the clock genes, mPer1, mPer2 and of the clock controlled gene, mDBP. However, the levels of each of these genes were significantly altered in different ways and recovered by feeding. We also found that feeding enhances phase-shifting to a new light-dark cycle of rhythmic mPer2 mRNA expression in the heart. Furthermore, feeding enhances the phase-shifting to new light-dark cycle of behaviour more than fasting. Our data indicate that feeding is an important cue for circadian behaviour rhythms as well as for the photo-entrainment of peripheral clock gene expression.