Paeoniflorin attenuates chronic constriction injury-induced neuropathic pain by suppressing spinal NLRP3 inflammasome activation
Paeoniflorin attenuates chronic constriction injury-induced neuropathic pain by suppressing spinal NLRP3 inflammasome activation
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DOI：
10.1007/s10787-020-00737-z
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发表时间：
2020-06-28
影响因子：
5.8
通讯作者：
Zhou, Jianyu
中科院分区：
文献类型：
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作者：
Liu, Pei;Cheng, Jianjun;Zhou, Jianyu
神经性疼痛仍然是全世界最常见的疼痛状况之一。越来越多的证据表明，NOD 样受体蛋白 3 (NLRP3) 炎症小体的激活有助于神经病理性疼痛的发病机制，尽管 NLRP3 炎症小体在神经病理性疼痛中的作用尚未完全阐明。在神经性疼痛的动物模型中，芍药苷（PF）被证明具有镇痛、抗炎和抗抑郁作用。然而，NLRP3 炎症小体在 PF 镇痛特性中的作用尚未研究。在本研究中，我们旨在确认脊髓中 NLRP3 炎性体的激活是否与神经性疼痛的发生有关，以及 PF 是否可以有效治疗此类疼痛。我们发现NLRP3炎性体的激活介导坐骨神经慢性压迫损伤后神经性疼痛的发展，并且PF通过抑制NLRP3炎性体激活来减轻神经性疼痛。此外，PF 还能增强转录因子核因子红细胞 2 相关因子 2 易位到细胞核中，并抑制脊髓中的核因子-κ B 活性。这些结果表明 PF 可能是神经性疼痛的潜在治疗剂。
Neuropathic pain remains one of the most common pain conditions worldwide. Accumulating evidence shows that activation of the NOD-like receptor protein 3 (NLRP3) inflammasome contributes to the pathogenesis of neuropathic pain, although the role of the NLRP3 inflammasome in neuropathic pain has not yet been fully elucidated. In animal models of neuropathic pain, paeoniflorin (PF) was shown to have analgesic, anti-inflammatory, and antidepressant effects. However, the role of the NLRP3 inflammasome in the analgesic properties of PF has not yet been studied. In this study, we aimed to confirm whether activation of the NLRP3 inflammasome in the spinal cord was involved in the development of neuropathic pain and whether PF could be an effective treatment for this type of pain. We found that activation of the NLRP3 inflammasome mediated the development of neuropathic pain following chronic constriction injury of the sciatic nerve and that PF attenuated neuropathic pain by inhibiting NLRP3 inflammasome activation. Moreover, PF enhanced the translocation of the transcription factor nuclear factor erythroid 2-related factor 2 into the nucleus and suppressed nuclear factor-kappa B activity in the spinal cord. These results suggest that PF may be a potential therapeutic agent for neuropathic pain.