Isolation and genetic analysis of human origin H1N1 and H3N2 influenza viruses from pigs in China
Isolation and genetic analysis of human origin H1N1 and H3N2 influenza viruses from pigs in China
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DOI：
10.1016/j.bbrc.2007.02.096
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发表时间：
2007-04-27
影响因子：
3.1
通讯作者：
Tong, Guang-Zhi
中科院分区：
文献类型：
--
作者：
Yu, Hai;Zhang, Gui-Hong;Tong, Guang-Zhi
甲型流感病毒H1N1和H3N2亚型在猪中已被广泛报道，与临床疾病相关。这些病毒主要包括典型的猪H1N1、类禽H1N1和类人或类禽H3N2病毒。从2005年到2006年，我们在中国的八个省份进行了猪流感病毒监测。在这里，我们报告，第一次，从中国南方广东省的一个农场的猪，一个主机怀疑通过基因重配产生新的大流行株的类似人的流感H1N1病毒的分离和遗传分析。这八个基因片段中的每一个都来自人类。系统发生分析表明，这些基因形成了人类谱系，表明该病毒是最近人类HIN I流感病毒的后代。此外，还分离到4株猪H3N2病毒。来自广东省的三种H3N2病毒是最近人类病毒的后代，而来自黑龙江省的H3N2病毒则来自早期人类病毒。通过对中国猪源人H1N1和H3N2流感病毒的分离和基因分析，进一步证实了人流感病毒在猪种间的传播，强调了加强猪流感病毒监测的重要性。(c)2007爱思唯尔公司All rights reserved.
Influenza A viruses of subtypes H1N1 and H3N2 have been reported widely in pigs, associated with clinical disease. These mainly include classical swine H1N1, avian-like H1N1, and human-like or avian-like H3N2 viruses. From 2005 to 2006, we carried out swine influenza virus surveillance in eight provinces of China. Here we report, for the first time, the isolation and genetic analysis of a human-like influenza H1N1 virus from a pig in a farm of Guangdong province of southern China, a host suspected to generate new pandemic strains through genetic reassortment. Each of the eight gene segments is of human origin. Phylogenetic analysis indicates that these genes form a human lineage, suggesting that this virus is the descendant of recent human H IN I influenza viruses. In addition, four swine H3N2 viruses were also isolated. The three H3N2 viruses from Guangdong province are descendants of recent human viruses, while an H3N2 virus from Heilongjiang province derives from early human viruses. Isolation and genetic analysis of human H1N1 and H3N2 influenza viruses from pigs in China provides further evidence about the interspecies transmission of human influenza viruses to pigs and emphasizes the importance of reinforcing swine influenza virus surveillance. (c) 2007 Elsevier Inc. All rights reserved.