Orthotopic transplantation of decellularized liver scaffold in mice.
Orthotopic transplantation of decellularized liver scaffold in mice.
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DOI：
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发表时间：
2015-01
影响因子：
0.1
通讯作者：
Hongyu Zhang;Yujun Zhang;F. Ma;P. Bie;L. Bai
中科院分区：
文献类型：
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作者：
Hongyu Zhang;Yujun Zhang;F. Ma;P. Bie;L. Bai
终末期肝病是一种威胁全世界数百万人生命的健康问题。原位肝移植是目前唯一的根治性治疗方法。然而，供体器官的持续短缺限制了患者接受这种治疗的机会。肝组织工程旨在通过产生用于治疗患有终末期肝病的个体的功能性组织构建体来克服这种限制。近年来，利用天然器官支架作为肝组织工程载体的新策略应运而生。这涉及含有天然器官系统的循环框架的脱细胞支架的制备。目前，在小实验动物中，特别是在小鼠中（mLBST），主要血管端侧吻合的肝脏支架移植的手术性能在技术上仍然具有挑战性。在这里，我们描述了mLBST的手术技术，可用于评估受体的工程肝移植。
End-stage liver disease is a life threatening health problem to millions of people worldwide. Orthotopic liver transplantation is the only therapy for the definitive cure at the present time. However, persistent shortage in donor organs limits the opportunity for patients to receive this treatment. Liver tissue engineering aims to overcome this restriction by generating functional tissue constructs for treatment of individuals with the end-stage liver disease. Recently, a new strategy has emerged using the natural organ scaffold as a vehicle for liver tissue engineering. This involves preparation of decellularized scaffold containing the circulatory framework of the natural organ system. Currently, surgical performance of liver scaffold transplantation with end-to-side anastomosis of major vessels in small experimental animals, particularly in mice (mLBST), remains technically challenging. Here, we describe surgical techniques of mLBST that can be used for evaluation of engineered liver grafts in recipients.