Expression of regulator of G-protein signalling protein 2 (RGS2) in the mouse uterus at implantation sites
Expression of regulator of G-protein signalling protein 2 (RGS2) in the mouse uterus at implantation sites
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DOI：
10.1530/rep.0.1260309
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发表时间：
2003-09-01
期刊：
影响因子：
3.8
通讯作者：
Shen, QX
中科院分区：
文献类型：
--
作者：
Huang, ZP;Ni, H;Shen, QX
在母体子宫内膜和植入的胚泡之间建立积极的对话是成功着床的关键。本研究的目的是通过消减杂交鉴定小鼠子宫着床部位明确表达的基因。所鉴定的基因的一个表达序列标签显示与G蛋白信号蛋白2（RGS 2）的调节剂具有92%的同一性。通过PCR扩增该基因的cDNA全序列，并在GenBank中注册。其开放阅读框编码211个氨基酸的序列与小鼠RGS 2的相同，除了4个氨基酸之外。北方印迹分析表明，该基因的表达在着床点比在着床间的网站在怀孕的第5-8天。原位杂交定位这种mRNA主要是在植入部位的基质细胞。这些结果表明，RGS 2在植入过程中的作用，可能通过调节细胞内Ca 2+动员和T细胞增殖的母胎界面。
Establishment of an active dialogue between the maternal endometrium and the implanting blastocyst is essential for successful implantation. The aim of this study was to identify genes that are explicitly expressed at implantation sites of the mouse uterus by subtractive hybridization. One expressed sequence tag of the genes identified showed 92% identity to the regulator of G-protein signalling protein 2 (RGS2). The full cDNA sequence of this gene was amplified by PCR and subsequently registered in GenBank. The sequence of its open reading frame encoding 211 amino acids was the same as that of mouse RGS2, with the exception of four amino acids. Northern blot analysis showed that the expression of this gene was much higher at implantation sites than at inter-implantation sites on days 5-8 of pregnancy. In situ hybridization localized this mRNA predominantly to the stromal cells at the implantation sites. These results indicate that RGS2 has a role during implantation, possibly by regulating the intracellular Ca2+ mobilization and T-cell proliferation at the maternal-fetal interface.