Encapsulation of mono- and oligo-nucleotides into aqueous-core nanocapsules in presence of various water-soluble polymers
Encapsulation of mono- and oligo-nucleotides into aqueous-core nanocapsules in presence of various water-soluble polymers
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DOI：
10.1016/j.ijpharm.2006.10.031
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发表时间：
2007-03-01
影响因子：
5.8
通讯作者：
Couvreur, P.
中科院分区：
文献类型：
--
作者：
Hillaireau, H.;Le Doan, T.;Couvreur, P.
在之前的研究中，我们已经证明西多福韦（CDV）和叠氮胸苷-三磷酸（AZT-TP）在聚氰基丙烯酸酯异丁酯（PIBCA）水核纳米胶囊中被包裹得很差。这是由于这些小而亲水的分子通过纳米胶囊的薄聚合物壁迅速泄漏。在本研究中，我们选择了各种水溶性聚合物作为增加Mw的佐剂，并研究了它们对单核苷酸（CDV, AZT-TP）和寡核苷酸（ODN）在这些PIBCA水核纳米胶囊中的包裹的影响。我们在这里表明，阳离子聚合物（即聚乙亚胺（PEI）或壳聚糖）在纳米胶囊水室中的存在允许AZT-TP和ODN的成功包封。(c) 2006 Elsevier B.V.版权所有
In a previous study, we have shown that cidofovir (CDV) and azidothymidine-triphosphate (AZT-TP) were poorly encapsulated in poly(isobutylcyanoacrylate) (PIBCA) aqueous-core nanocapsules. This was attributed to the rapid leakage of these small and hydrophilic molecules through the thin polymer wall of the nanocapsules. In the present study, we have selected various water-soluble polymers as increasing Mw adjuvants and investigated their influence on the entrapment of mononucleotides (CDV, AZT-TP) as well as of oligonucleotides (ODN) into these PIBCA aqueous-core nanocapsules. We show here that the presence of cationic polymers (i.e. poly(ethyleneimine) (PEI) or chitosan) in the nanocapsule aqueous compartment allowed successful encapsulation of AZT-TP and ODN. (c) 2006 Elsevier B.V. All rights reserved.