Human antibody to OFA-I, a tumor antigen, produced in vitro by Epstein-Barr virus-transformed human B-lymphoid cell lines.
Human antibody to OFA-I, a tumor antigen, produced in vitro by Epstein-Barr virus-transformed human B-lymphoid cell lines.
复制标题
OFA-I（一种肿瘤抗原）的人抗体，由 Epstein-Barr 病毒转化的人 B 淋巴细胞系在体外产生。
DOI：
10.1073/pnas.79.18.5666
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发表时间：
1982
影响因子：
11.1
通讯作者：
Saxton,RE
中科院分区：
文献类型：
--
作者：
Irie,RF;Sze,LL;Saxton,RE
我们建立了两个长期的人b淋巴母细胞样细胞系（L55和L72），由eb病毒转化，产生针对人肿瘤抗原OFA-I的IgM抗体。B淋巴细胞来源于黑色素瘤患者的外周血淋巴细胞。抗体特异性是通过免疫粘附试验确定的，使用各种人类癌症和非癌症组织作为靶点。L55抗体（指定抗ofa - i- 1）与多种人类肿瘤类型反应，而L72抗体（指定抗ofa - i- 2）仅与神经外胚层来源的肿瘤细胞（黑色素瘤、胶质瘤和神经母细胞瘤）反应。放射免疫法检测1 × 10(6) L55和L72细胞废液中IgM水平分别为4和9微克/ml。
We established two long-term human B-lymphoblastoid cell lines (L55 and L72) transformed by Epstein-Barr virus that produced IgM kappa antibodies to the human tumor antigen, OFA-I. Periphral blood lymphocytes obtained from melanoma patients were used as the source of the B lymphocytes. Antibody specificity was determined by the immune adherence assay using various human cancer and noncancer tissues as targets. L55 antibody (designated anti-OFA-I-1) reacted with a variety of human tumor types whereas L72 antibody (designated anti-OFA-I-2) reacted only with tumor cells of neuroectodermal origin (melanoma, glioma, and neuroblastoma). The levels of IgM detected in the spent medium of 1 X 10(6) L55 and L72 cells were 4 and 9 micrograms/ml, respectively, by radioimmunoassay.