Single-cell Transcriptome Profiling reveals Dermal and Epithelial cell fate decisions during Embryonic Hair Follicle Development
Single-cell Transcriptome Profiling reveals Dermal and Epithelial cell fate decisions during Embryonic Hair Follicle Development
复制标题
单细胞转录组分析揭示胚胎毛囊发育过程中真皮和上皮细胞的命运决定
DOI：
10.7150/thno.44306
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发表时间：
2020-01-01
期刊：
影响因子：
12.4
通讯作者：
Wang, Xin
中科院分区：
文献类型：
--
作者：
Ge, Wei;Tan, Shao-Jing;Wang, Xin
毛囊发育过程中形态结构的表征已经有了很好的文献记载，而目前对毛囊发育的分子机制的理解仍然有限。在这里，我们使用无偏单细胞RNA测序，分析了来自E13.5和E16.5胎鼠和新生小鼠（出生后0天，P0）背部皮肤细胞的15,086个单细胞转录组谱。基于t分布随机邻居嵌入（tSNE）聚类，我们从皮肤细胞中鉴定了14个细胞簇，并描绘了它们的细胞身份基因表达谱。伪时间排序分析成功构建了上皮/真皮细胞谱系分化轨迹，揭示了细胞命运决定过程中关键调控的顺序激活。与此同时，基于对配体-受体对的先验知识推断了不同细胞群之间的细胞间通信。总之，我们的研究结果提供了毛囊上皮/真皮细胞谱系命运决定过程中的分子景观，更重要的是，概述了毛囊形态发生过程中不同细胞群的核心调控转录因子的顺序激活。
Characterization of the morphological structure during hair follicle development has been well documented, while the current understanding of the molecular mechanisms involved in follicle development remain limited. Here, using unbiased single-cell RNA sequencing, we analyzed 15,086 single cell transcriptome profiles from E13.5 and E16.5 fetal mice, and newborn mouse (postnatal day 0, P0) dorsal skin cells. Based on t-distributed Stochastic Neighbor Embedding (tSNE) clustering, we identified 14 cell clusters from skin cells and delineated their cell identity gene expression profiles. Pseudotime ordering analysis successfully constructed epithelium/dermal cell lineage differentiation trajectory and revealed sequential activation of key regulons involved during cell fate decisions. Along with this, intercellular communication between different cell populations were inferred based on a priori knowledge of ligand-receptor pairs. Together, our findings here provide a molecular landscape during hair follicle epithelium/dermal cell lineage fate decisions, and more importantly, recapitulate sequential activation of core regulatory transcriptional factors for different cell populations during hair follicle morphogenesis.