NUCLEOTIDE-SEQUENCE ANALYSIS OF THE PHOSPHOMANNOSE ISOMERASE GENE (PMI) OF PSEUDOMONAS-AERUGINOSA AND COMPARISON WITH THE CORRESPONDING ESCHERICHIA-COLI GENE MANA
NUCLEOTIDE-SEQUENCE ANALYSIS OF THE PHOSPHOMANNOSE ISOMERASE GENE (PMI) OF PSEUDOMONAS-AERUGINOSA AND COMPARISON WITH THE CORRESPONDING ESCHERICHIA-COLI GENE MANA
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DOI：
10.1016/0378-1119(86)90233-7
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发表时间：
1986-01-01
期刊：
影响因子：
3.5
通讯作者：
CHAKRABARTY, AM
中科院分区：
文献类型：
--
作者：
DARZINS, A;FRANTZ, B;CHAKRABARTY, AM
磷酸甘露糖异构酶(PMI)被认为是铜绿假单胞菌褐藻酸生物合成途径的第一步。测定了2.0kb的BamHI-SSTI DNA片段上的铜绿假单胞菌Pmi基因的核苷酸序列。该基因由起始密码子和终止密码子以及与预测MR为52860的多肽产物相对应的1440bp的开放阅读框在体外被破坏而确定。该多肽表现出明显的MR约为。56000麦氏提取液在十二烷基硫酸钠-聚丙烯酰胺凝胶上的电泳法。Pmi基因的密码子利用受到铜绿假单胞菌DNA高G+C含量(66mol%)的影响。计算机辅助匹配分析未能证明铜绿假单胞菌PMI和大肠杆菌MANA(PMI)基因在核苷酸水平上有任何显著的同源性。然而，在其他假单胞菌中发现了与铜绿假单胞菌Pmi基因同源的序列，如恶臭假单胞菌和孟氏假单胞菌，以及能够产生藻酸的棕色固氮杆菌。
Phosphomannose isomerase (PMI) has been proposed to catalyze the first step of the alginic acid biosynthetic pathway in Pseudomonas aeruginosa. The nucleotide sequence of the P. aeruginosa pmi gene contained on a 2.0-kb BamHI-SstI DNA fragment has been determined. The gene was defined by the start and stop codons and by in vitro disruption of an open reading frame of 1440 bp corresponding to a polypeptide product with a predicted Mr of 52860. This polypeptide displayed an apparent Mr of approx. 56000 upon electrophoresis of a maxicell extract on sodium dodecyl sulfate-polyacrylamide gels. The codon utilization of the pmi gene was distinct in the wobble base preference and influenced by the high G + C content (66 mol%) of the P. aeruginosa DNA. Computer assisted matching analysis failed to demonstrate any significant homology at the nucleotide level between the P. aeruginosa pmi and Escherichia coli manA (pmi) genes. However, sequences homologous to the P. aeruginosa pmi gene were found in other Pseudomonas species, such as P. putida and P. mendocina, and in Azotobacter vinelandii, all capable of producing alginic acid.