Neural cell adhesion molecule expression in Xenopus embryos.
Neural cell adhesion molecule expression in Xenopus embryos.
复制标题
非洲爪蟾胚胎中神经细胞粘附分子的表达。
DOI：
10.1016/0012-1606(87)90057-1
复制
发表时间：
1987
影响因子：
2.7
通讯作者：
Rutishauser,U
中科院分区：
文献类型：
--
作者：
Balak,K;Jacobson,M;Sunshine,J;Rutishauser,U
神经细胞粘附分子NCAM的表达的时空模式映射在胚胎的蛙爪蟾，从囊胚到早期游泳阶段，使用多克隆抗体，识别XenopusNCAM。神经板期是NCAM可被检测到的最早期。NCAM免疫反应的起始部位是神经外胚层、体细胞中胚层和脊索中胚层。在神经管的形成过程中，NCAM免疫反应性仅限于神经外胚层及其衍生物。在神经管关闭期间以及此后2-4小时，NCAM在神经管的冠状切片中以独特的放射状模式表达。NCAM在迁移前和颅神经节和脊神经节形成后的神经嵴细胞中观察到。在最初的神经突起生长期间，NCAM集中在发育中的中枢神经纤维通路中。NCAM在周围神经上从它们最初的生长开始就被观察到，并且在它们形成期间在神经肌肉接头处强烈表达。这些结果表明，NCAM的表达后，神经诱导和功能的神经板和管，一些神经嵴衍生物，神经纤维束的发展，形成神经肌肉连接的形态发生。
The spatiotemporal pattern of expression of the neural cell adhesion molecule NCAM was mapped immunohistochemically in embryos of the frogXenopus, from blastula to early swimming stages, using a polyclonal antibody that recognizesXenopusNCAM. The neural plate stage was the earliest at which NCAM could be detected. The initial sites of NCAM immunoreactivity were neural ectoderm, somitic mesoderm, and chordamesoderm. During formation of the neural tube, NCAM immunoreactivity became restricted to the neuroectoderm and its derivatives. During closure of the neural tube and for 2–4 hr thereafter, NCAM was expressed in a distinctive radial pattern in coronal sections of the neural tube. NCAM was observed in neural crest cells before migration and after formation of cranial and spinal ganglia. During the period of initial neurite outgrowth, NCAM became concentrated in the developing central nerve fiber pathways. NCAM was seen on peripheral nerves from the time of their initial outgrowth and it was strongly expressed at neuromuscular junctions during the period of their formation. These results show that NCAM is expressed after neural induction and functions during morphogenesis of the neural plate and tube, some neural crest derivatives, development of nerve fiber tracts, and formation of neuromuscular connections.