Complex community of nitrite-dependent anaerobic methane oxidation bacteria in coastal sediments of the Mai Po wetland by PCR amplification of both 16S rRNA and pmoA genes
Complex community of nitrite-dependent anaerobic methane oxidation bacteria in coastal sediments of the Mai Po wetland by PCR amplification of both 16S rRNA and pmoA genes
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DOI：
10.1007/s00253-014-6051-6
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发表时间：
2015-02-01
影响因子：
5
通讯作者：
Gu, Ji-Dong
中科院分区：
文献类型：
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作者：
Chen, Jing;Zhou, Zhichao;Gu, Ji-Dong
本工作利用16SRRNA和pmoA基因为基础的聚合酶链式反应，成功地研究了米埔滨海湿地表层和下层沉积物中n-达摩细菌的多样性和分布。本研究证实了表层和地下沉积物中均存在多样性较高的n-达摩细菌。与沿海已知的两个n-达摩群落不同，本工作中的pmoA基因扩增序列不仅与一些淡水亚群聚在一起，而且大多聚集在新建立的三个海洋亚群内，表明了该湿地中n-达摩细菌独特的生态位特异性。结果表明，植被影响了沉积物中n-达摩细菌的分布和群落结构，在海岸生态系统中，n-达摩细菌可以与硫酸盐还原甲氧菌共存。基于16SRRNA基因的米埔n-达摩细菌的群落结构与淡水和海水的不同。相反，基于pmoA基因的米埔n-达摩群落的结构与海洋群落聚在一起，并与淡水群落有明显的区别。用16SRRNA基因聚合酶链式反应对该湿地n-达摩细菌的丰度进行了量化，结果为2.65-6.71×10(5)拷贝/g干沉积物。氨氮和亚硝酸盐强烈影响米埔滨海湿地沉积物中n-达摩细菌的群落结构和分布。
In the present work, both 16S rRNA and pmoA gene-based PCR primers were employed successfully to study the diversity and distribution of n-damo bacteria in the surface and lower layer sediments at the coastal Mai Po wetland. The occurrence of n-damo bacteria in both the surface and subsurface sediments with high diversity was confirmed in this study. Unlike the two other known n-damo communities from coastal areas, the pmoA gene-amplified sequences in the present work clustered not only with some freshwater subclusters but also within three newly erected marine subclusters mostly, indicating the unique niche specificity of n-damo bacteria in this wetland. Results suggested vegetation affected the distribution and community structures of n-damo bacteria in the sediments and n-damo could coexist with sulfate-reducing methanotrophs in the coastal ecosystem. Community structures of the Mai Po n-damo bacteria based on 16S rRNA gene were different from those of either the freshwater or the marine. In contrast, structures of the Mai Po n-damo communities based on pmoA gene grouped with the marine ones and were clearly distinguished from the freshwater ones. The abundance of n-damo bacteria at this wetland was quantified using 16S rRNA gene PCR primers to be 2.65-6.71 x 10(5) copies/g dry sediment. Ammonium and nitrite strongly affected the community structures and distribution of n-damo bacteria in the coastal Mai Po wetland sediments.