A NOVEL MEMBER OF THE THYROID STEROID-HORMONE RECEPTOR FAMILY IS ENCODED BY THE OPPOSITE STRAND OF THE RAT C-ERBA-ALPHA TRANSCRIPTIONAL UNIT
A NOVEL MEMBER OF THE THYROID STEROID-HORMONE RECEPTOR FAMILY IS ENCODED BY THE OPPOSITE STRAND OF THE RAT C-ERBA-ALPHA TRANSCRIPTIONAL UNIT
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DOI：
10.1128/mcb.9.3.1128
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发表时间：
1989-03-01
影响因子：
5.3
通讯作者：
CHIN, WW
中科院分区：
文献类型：
--
作者：
LAZAR, MA;HODIN, RA;CHIN, WW
从大鼠GH3细胞文库中分离到一个编码甲状腺/类固醇激素受体超家族新成员的基因，称为Rev-ErbAα。Rev-ErbA.Alpha.是一种大约56千道尔顿的蛋白质，在结构上与甲状腺激素受体(c-ERBA)和维甲酸受体最相似，但它既不结合甲状腺激素，也不结合维甲酸。编码Rev-ErbAα的mRNA。存在于许多组织中，尤其在骨骼肌和棕色脂肪中含量丰富。包含完整Rev-ErbAα的基因组DNA片段。分离并鉴定了该基因的cDNA序列。值得注意的是，这个DNA片段还包含c-erbAα的一部分。基因r-erb Aα-1和r-erbAα-2是c-erb Aα的选择性剪接产物。基因和是受体超家族的成员。编码Rev-ErbAα的基因。和r-erbAα-2重叠，它们的编码链彼此相对。双向转录区域的269个碱基对片段在REV-ErbA.pha中都是外显子。和r-erbAα-2基因，产生互补的mRNAs。因此，通过选择性剪接和反链转录，一个基因组位点编码甲状腺/类固醇激素受体超家族的三个不同成员。讨论了这种不寻常的基因组排列的潜在含义。
A cDNA encoding a novel member of the thyroid/steroid hormone receptor superfamily, called Rev-ErbA.alpha., has been isolated from a rat GH3 cell library. Rev-ErbA.alpha. is an .apprx.56-kilodalton protein most similar in structure to the thyroid hormone receptor (c-erbA) and the retinoic acid receptor, but it does not bind either thyroid hormone or retinoic acid. The mRNA encoding Rev-ErbA.alpha. is present in many tissues and is particularly abundant in skeletal muscle and brown fat. A genomic DNA fragment containing the entire Rev-ErbA.alpha. cDNA sequence was isolated and characterized. Remarkably, this DNA fragment also contained a portion of the c-erbA.alpha. gene, r-erbA.alpha.-1 and r-erbA.alpha.-2 are alternative splice products of the c-erbA.alpha. gene and are members of the receptor superfamily. The genes encoding Rev-ErbA.alpha. and r-erbA.alpha.-2 overlap, with their coding strands oriented opposite one another. A 269-base-pair segment of the bidirectionally transcribed region is exonic in both the Rev-ErbA.alpha. and r-erbA.alpha.-2 genes, resulting in complementary mRNAs. Thus, through alternative splicing and opposite-strand trancription, a single genomic locus codes for three different members of the thyroid/steroid hormone receptor superfamily. Potential implications of this unusual genomic arrangement are discussed.