Expression of estrogen receptors α and β in human lung tissue and cell lines
Expression of estrogen receptors α and β in human lung tissue and cell lines
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DOI：
10.1016/s0169-5002(02)00039-9
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发表时间：
2002-08-01
期刊：
影响因子：
5.3
通讯作者：
Haugen, A
中科院分区：
文献类型：
--
作者：
Mollerup, S;Jorgensen, K;Haugen, A
流行病学研究表明，与男性相比，女性患吸烟相关肺癌的风险更高。一些生物化学证据支持这些观察结果。循环类固醇激素在肺癌病因学中的可能作用已被假设。在本文中，我们研究了组织学正常的人肺组织和肺肿瘤细胞系中雌激素受体(ER)- α和erβ的表达。逆转录- pcr检测相对ER mRNA水平，并将其归一化为甘油醛3-磷酸脱氢酶基因（GAPDH）的表达水平。在肺组织中，46例病例中有38例（83%）发现不同水平的erα转录物。所有病例均表达ERbeta。雌激素受体在雌性和雄性中的表达水平相似，erα和erβ mRNA的表达水平显著相关(P
Epidemiological studies have indicated that females may be at greater risk of smoking associated lung cancer compared with males. Several lines of biochemical evidence support these observations. A possible role of circulating steroid hormones in the etiology of lung cancer has been hypothesized. In the present paper, we have studied the expression of the estrogen receptors (ER)-alpha and ERbeta in histologically normal human lung tissue and lung tumor cell lines. Relative ER mRNA levels were measured by reverse transcriptase-PCR and normalized to the level of expression of the glyceraldehyde 3-phosphate dehydrogenase gene (GAPDH). In lung tissue, an ERalpha transcript was found at various levels in 38 out of 46 cases (83%). ERbeta was expressed in all cases. The ERs were expressed at similar levels in females and males, and the levels of ERalpha and ERbeta mRNA were significantly related (P