An unusually compact external promoter for RNA polymerase III transcription of the human H1RNA gene
An unusually compact external promoter for RNA polymerase III transcription of the human H1RNA gene
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DOI：
10.1093/nar/29.12.2502
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发表时间：
2001-06-15
影响因子：
14.9
通讯作者：
Carbon, P
中科院分区：
文献类型：
--
作者：
Myslinski, E;Amé, JC;Carbon, P
H1RNA是人核RNaseP的RNA组分，由RNA聚合酶III(Pol III)转录的一个独特基因编码。本工作通过突变模板的转录分析和重组蛋白的DNA分析，对参与人类H1基因转录的顺式作用元件和反式作用因子进行了鉴定。在5‘-侧翼序列的100bp范围内，有四个元件被定义为在体外和体内最大限度地表达所必需的元件，包括八聚体、STAF、近端序列元件(PSE)和TATA基序。这些也存在于脊椎动物SnRNA基因的启动子元件中，其中前两个组成了末端序列元件(DSE)。到目前为止，在所有被检测的基因中，DSE距离组成基本启动子的PSE和TATA盒都很远。然而，我们观察到H1RNA和SnRNA基因启动子的组织结构相对于DSE和PSE的相对间距有根本的不同。事实上，H1启动子异常紧凑，八聚体基序和STAF结合位点邻近PSE和TATA基序。因此，人类RNase P RNA基因似乎采用了一种独特的启动子策略，将DSE直接放置在基本启动子附近。
H1 RNA, the RNA component of the human nuclear RNase P, is encoded by a unique gene transcribed by RNA polymerase III (Pol III). In this work, cis-acting elements and trans-acting factors involved in human H1 gene transcription were characterized by transcription assays of mutant templates and DNA assays of recombinant proteins. Four elements, lying within 100 bp of 5'-flanking sequences, were defined to be essential for maximal in vitro and in vivo expression, consisting of the octamer, Staf, proximal sequence element (PSE) and TATA motifs. These are also encountered in the promoter elements of vertebrate snRNA genes, where the first two constitute the distal sequence element (DSE). In all the genes examined so far, the DSE is distant from the PSE and TATA box that compose the basal promoter. However, we observed a fundamental difference in the organization of the H1 RNA and snRNA gene promoters with respect to the relative spacing of the DSE and PSE. Indeed, the H1 promoter is unusually compact, with the octamer motif and Staf binding site adjacent to the PSE and TATA motifs. It thus appears that the human RNase P RNA gene has adopted a unique promoter strategy placing the DSE immediately adjacent to the basal promoter.