Studies on sex-organ development. Prenatal effect of oestrogenic hormone on tubular-gland cell morphogenesis and ovalbumin-gene expression in the chick Müllerian duct.
Studies on sex-organ development. Prenatal effect of oestrogenic hormone on tubular-gland cell morphogenesis and ovalbumin-gene expression in the chick Müllerian duct.
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雌激素对雏鸡苗勒氏管中管状腺细胞形态发生和卵清蛋白基因表达的产前影响的研究。
作者：
G. Andrews;C. Teng
研究了己烯雌酚对鸡胚左苗勒管形态发生和细胞分化的影响。胚胎在不同的发展阶段与最大反应剂量的己烯雌酚超过5天的时间间隔。然后测定苗勒氏管的壳腺和枕骨大区的生长和组织形态发生。结果与己烯雌酚诱导的卵清蛋白基因表达的卵清蛋白-mRNA（mRNAov）积累和卵清蛋白合成的相对速率测量。从孵育第10天到第15天处理胚胎可诱导苗勒管大管内管状腺细胞的形态发生。虽然这些细胞占总细胞群的10%，每个细胞平均含有8000个mRNAov分子，但卵清蛋白合成仅占总magnum蛋白合成的0.85%。从孵育第13天到第18天处理的胚胎的苗勒管大管含有约30%的管状腺细胞，其平均每个细胞积累了7000个mRNAov分子，但卵清蛋白合成仅占总大管蛋白合成的3.25%。从孵育第16天到第21天处理的胚胎的苗勒管大管含有约50%的管状腺细胞，其平均每个细胞积累了6500个mRNAov分子，卵清蛋白合成占总大管蛋白合成的10%。雌激素反应性在苗勒管大管和壳腺区域同时发生。与己烯雌酚诱导的输卵管生长速率相比，己烯雌酚诱导的苗勒管生长的相对速率随着胚胎年龄的增加而增加，从10天胚胎的20倍降低到16天胚胎的3倍。所有的结果进行了讨论，在比较与雌激素的未成熟的鸡输卵管，并在个体发育的胚胎主管上皮和基质成分的苗勒管。结论：胚胎苗勒管雌激素活性的发育是一个多阶段的现象。苗勒管中激素反应性的急剧增加发生在发育的第10天和第16天之间，雌激素反应性的最终成熟发生在发育的第16天和孵化后1周之间。
The effects of diethylstilboestrol on morphogenesis and cyto-differentiation of the chick-embryo left Müllerian duct were examined. Embryos were treated at different stages of development with maximal-responsive doses of diethylstilboestrol over a 5-day interval. The shell gland and magnum regions of the Müllerian duct were then assayed for growth and histological morphogenesis. The results were correlated with diethylstilboestrol-induced ovalbumin-gene expression as measured by ovalbumin-mRNA (mRNAov) accumulation and the relative rate of ovalbumin synthesis. Treatment of the embryo from day 10 to day 15 of incubation induces morphogenesis of tubular-gland cells in the Müllerian-duct magnum. Although these cells constitute 10% of the total cell population and contain an average of 8000 molecules of mRNAov per cell, ovalbumin synthesis is only 0.85% of total magnum protein synthesis. The Müllerian-duct magnum of embryos treated from day 13 to day 18 of incubation contains about 30% tubular-gland cells, which have accumulated an average of 7000 molecules of mRNAov per cell, but ovalbumin synthesis is only 3.25% of total magnum protein synthesis. The Müllerian-duct magnum of embryos treated from day 16 to day 21 of incubation contains about 50% tubular-gland cells, which have accumulated an average of 6500 mRNAov molecules per cell, and ovalbumin synthesis is 10% of total magnum protein synthesis. Oestrogen responsiveness develops simultaneously in the Müllerian-duct magnum and shell-gland regions. Compared with the rate of diethylstilboestrol-induced oviduct growth, the relative rate of diethylstilboestrol-induced Müllerian-duct growth increases with embryonic age, from 20-fold lower in the 10-day embryo to only 3-fold lower in the 16-day embryo. All results are discussed in comparison with the responses to oestrogen of the immature chick oviduct, and in terms of the ontogeny of hormone-competent epithelial and stromal components of the Müllerian duct. It is concluded that the development of oestrogenic competence in the embryonic Müllerian duct is a multiphasic phenomenon. A dramatic increase in hormone responsiveness in the Müllerian duct occurs between days 10 and 16 of development, and a less dramatic final maturation of oestrogen responsiveness occurs between day 16 of development and 1 week after hatching.