Entomopathogenic Beauveria bassiana granules to control soil-dwelling stage of western flower thrips, Frankliniella occidentalis (Thysanoptera: Thripidae)
Entomopathogenic Beauveria bassiana granules to control soil-dwelling stage of western flower thrips, Frankliniella occidentalis (Thysanoptera: Thripidae)
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DOI：
10.1007/s10526-017-9818-8
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发表时间：
2017-10-01
期刊：
影响因子：
2.5
通讯作者：
Kim, Jae Su
中科院分区：
文献类型：
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作者：
Lee, Se Jin;Kim, Sihyeon;Kim, Jae Su
西花蓟马Frankliniella occurentalis（Pergande）是一种世界性的重要害虫，化学农药的过度使用造成了环境污染和害虫的抗药性。本文报道了利用昆虫病原球孢白僵菌颗粒剂防治土壤中的西花蓟马。使用B。表达增强型绿色荧光蛋白（Bb-egfp）的球孢白僵菌分离物，我们证实Bb-egfp对蓟马具有高度毒性并定殖于土壤，这允许土壤中的腐殖菌与定殖的真菌块接触。在温室试验中，将BbERL 836和BbERL 1578分离物的颗粒施用于盆中生长的番茄的土壤表面，并且雌性F.在施用真菌三天后，在番茄植物上释放ococculentalis。在施用后20天和40天，在BbERL 836和BbERL 1578的处理中观察到75%至90%的种群减少（化学控制：85%减少）。在黄瓜田间试验中，两种ERL土壤处理均表现出较好的增产效果。90%的防治效果，与半合成杀虫剂多杀菌素（85%的防治效果）相似。本研究表明，利用昆虫病原B。球孢白僵菌颗粒剂对土壤中的蓟马是一种有效的防治措施。番茄和黄瓜中的occurentalis种群。
The western flower thrip, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae), is recognized as a serious pest worldwide, but overuse of chemical pesticides results in environmental pollution and insect resistance. Herein we report the biocontrol of soil-dwelling stages of western flower thrips using entomopathogenic Beauveria bassiana granules. Using a B. bassiana isolate expressing enhanced green fluorescent protein (Bb-egfp), we confirmed that Bb-egfp was highly virulent to the thrips and colonized the soil, which allowed soil-dwelling pupae to come in contact with the colonized fungal mass. In a glasshouse trial, granules of BbERL836 and BbERL1578 isolates were applied to the soil surface of tomatoes growing in pots and female F. occidentalis were released on tomato plants three days after the fungal application. Populations reductions between 75 and 90% were observed in the treatments of BbERL836 and BbERL1578 at 20 and 40 days after application (chemical control: 85% reduction). In the field trial with cucumbers, the two ERL soil treatments showed ca. 90% control efficacy, similar to that of spinetorm, a semi-synthetic insecticide (85% efficacy). This study shows that using entomopathogenic B. bassiana granules to kill soil-dwelling stages of thrips is an effective strategy to manage F. occidentalis populations in tomato and cucumber.