Characterization of dextran transport and molecular weight cutoff (MWCO) of large pore size hollow fiber ultrafiltration membranes
Characterization of dextran transport and molecular weight cutoff (MWCO) of large pore size hollow fiber ultrafiltration membranes
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DOI：
10.1016/j.memsci.2020.119025
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发表时间：
2021-03
影响因子：
9.5
通讯作者：
Christopher J. Yehl;A. Zydney
中科院分区：
文献类型：
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作者：
Christopher J. Yehl;A. Zydney
疫苗、基因治疗剂和病毒样颗粒的发展为在下游加工中使用大孔径超滤膜创造了令人兴奋的机会。膜生产商和最终用户面临的挑战之一是难以评估膜孔径或标称分子量切断（MWCO），以选择合适的膜进行目标分离。我们采用实验测量和理论建模相结合的方法，开发了一种基于葡聚糖保留测量的大孔径超滤膜MWCO的改进方法。该模型既考虑了浓度极化效应，也考虑了固有的葡聚糖保留行为。研究结果不仅提供了控制葡聚糖保留的基本因素，而且还可以指导膜制造商和最终用户选择最合适的条件（即进料流量和渗透通量）来评估具有不同几何形状、孔径和渗透率的中空纤维的MWCO。
The development of vaccines, gene therapy agents, and virus-like particles has created exciting opportunities for the use of large pore size ultrafiltration membranes in downstream processing. One of the challenges facing both membrane producers and end-users is the difficulty in evaluating the membrane pore size or nominal molecular weight cutoff (MWCO) for the selection of appropriate membranes for targeted separations. We have used a combination of experimental measurements and theoretical modeling to develop an improved methodology for evaluating the MWCO of these large pore size ultrafiltration membranes based on dextran retention measurements. The model accounts for both concentration polarization effects and the intrinsic dextran retention behavior. The results not only provide fundamental insights into the factors controlling dextran retention, but they can also guide membrane manufacturers and end-users to the most appropriate conditions (i.e. feed flow rate and permeate flux) for evaluating the MWCO for hollow fibers with different geometry, pore size, and permeability.