THE EFFECTS OF MONO-(2-ETHYLHEXYL)-PHTHALATE ON RAT SERTOLI CELL-ENRICHED PRIMARY CULTURES
THE EFFECTS OF MONO-(2-ETHYLHEXYL)-PHTHALATE ON RAT SERTOLI CELL-ENRICHED PRIMARY CULTURES
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DOI：
10.1016/0041-008x(88)90186-x
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发表时间：
1988-03-15
影响因子：
3.8
通讯作者：
DUTTON, SL
中科院分区：
文献类型：
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作者：
CHAPIN, RE;GRAY, TJB;DUTTON, SL
体内研究中有相当多的证据表明，支持细胞是邻苯二甲酸酯在啮齿动物睾丸中作用的初始靶细胞。由于代谢活跃的细胞类型在精子发生中起着中心作用，我们研究了有毒的邻苯二甲酸酯，单-（2-乙基己基）-邻苯二甲酸酯（MEHP），对18日龄大鼠睾丸细胞培养物的分泌和合成活性的影响。这些培养物是78-84%的支持细胞。暴露于MEHP降低细胞ATP约。20%，降低了放射性标记的14 CO2从乙酸盐的产生，并降低了丙酮酸盐的培养基水平，同时增加了乳酸盐和细胞内脂质的培养基水平。通过放射性标记的亮氨酸掺入评价的蛋白质合成不受MEHP的影响。线粒体琥珀酸脱氢酶活性降低MEHP的存在。Michaelis-Menton动力学分析表明，这是一种混合抑制作用。线粒体罗丹明123摄取无明显变化。这些数据与线粒体是支持细胞中MEHP作用的一个靶点的概念一致。
There is considerable evidence from in vivo studies that the Sertoli cell is an initial target cell for the actions of phthalates in the rodent testis. Because the metabolically active cell type plays a cental role in spermatogenesis, we examined the effects of a toxic phthalate, mono-(2-ethylhexyl)-phthalate (MEHP), on the secretory and synthetic activities of primary testicular cell cultures isolated from 18-day-old rats. These cultures were 78-84% Sertoli cells. Exposure to MEHP decreased cellular ATP by ca. 20%, decreased production of radiolabeled 14CO2 from acetate, and decreased media levels of pyruvate, while it increased media levels of lactate and intracellular lipid. Protein synthesis, evaluated by radiolabled leucine incorporation, was not affected by MEHP. Mitochondrial succinate dehydrogenase activity was decreased in the presence of MEHP. Michaelis-Menton kinetic analysis indicated this was a mixed inhibition. There was no apparent change in mitochondrial Rhodamine 123 uptake. These data are consistent with the concept that mitochondria are one target for the actions of MEHP in the Sertoli cell.