Temporal and differential regulation of expression of the eukaryotic-like ankyrin effectors of Legionella pneumophila
Temporal and differential regulation of expression of the eukaryotic-like ankyrin effectors of Legionella pneumophila
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DOI：
10.1111/j.1758-2229.2010.00159.x
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发表时间：
2010-10-01
影响因子：
3.3
通讯作者：
Abu Kwaik, Yousef
中科院分区：
文献类型：
--
作者：
Al-Khodor, Souhaila;Al-Quadan, Tasneem;Abu Kwaik, Yousef
当从生长的指数期(E)过渡到指数期后（PE）时，嗜肺军团菌经历了从复制型到高传染性的表型调节。这种转变需要一个微妙的调控级联，它被触发来诱导各种毒力相关基因的表达。我们最近鉴定了11个嗜肺乳杆菌真核样锚蛋白效应物（Ank），它们在嗜肺乳杆菌的4个测序基因组中共享。AnkB效应将多泛素化蛋白招募到含军团菌液泡（LCV）。目前尚不清楚在PE期触发的各种调控是否调控了ank基因的表达，以及这种调控是否对功能至关重要。本研究表明，RelA、增强子蛋白LetE以及LetA/S和PmrA/B两个组成系统介导了ank基因的时间和差异调控。与ankB在PE期的表达一致，我们发现生长到PE期而不是E期的细菌以ankB依赖的机制将多泛素化蛋白募集到棘阿米巴的LCV中。我们得出结论，编码嗜肺乳杆菌真核样Ank效应的基因在PE期受到时间和空间的调控。
P>Upon transition from the exponential (E) to the post-exponential phase (PE) of growth, Legionella pneumophila undergoes a phenotypic modulation from a replicative to a highly infectious form. This transition requires a delicate regulatory cascade that is triggered to induce expression of various virulence-related genes. We have recently characterized eleven L. pneumophila eukaryotic-like ankyrin effectors (Ank) shared between the four sequenced genomes of L. pneumophila. The AnkB effector recruits polyubiquitinated proteins to the Legionella-containing vacuole (LCV). It is not known whether expression of the ank genes is regulated by various regulators triggered at the PE phase and whether this regulation is essential for function. Here we show that temporal and differential regulation of the ank genes is mediated by RelA, the enhancer protein LetE, and the two component systems LetA/S and PmrA/B. Consistent with the expression of ankB at the PE phase, we show that bacteria grown to the PE but not the E phase recruit polyubiquitinated proteins to the LCV within Acanthamoeba in an AnkB-dependant mechanism. We conclude that the genes encoding the eukaryotic-like Ank effectors of L. pneumophila are temporally and spatially regulated at the PE phase.