Localization of topoisomerase II in mitotic chromosomes.
Localization of topoisomerase II in mitotic chromosomes.
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拓扑异构酶II在有丝分裂染色体中的定位。
DOI：
10.1083/jcb.100.5.1716
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发表时间：
1985-05
影响因子：
7.8
通讯作者：
Heck, M M
中科院分区：
文献类型：
--
作者：
Earnshaw, W C;Heck, M M
在之前的文章中，我们描述了一种识别cSc-1的多克隆抗体，cSc-1是鸡有丝分裂染色体支架的主要多肽成分。该多肽被证明是鸡拓扑异构酶II。在本文描述的实验中，我们使用间接免疫荧光和免疫电子显微镜来检查拓扑异构酶II在完整染色体内的分布。我们还描述了一个简单的实验方案，区分抗原，沿着染色质纤维穿插从那些占据限制区域在染色体内。这些实验表明，酶的分布似乎与大块染色质无关。我们的数据表明拓扑异构酶II与有丝分裂染色体径向环结构域的碱基结合。
In the preceding article we described a polyclonal antibody that recognizes cSc-1, a major polypeptide component of the chicken mitotic chromosome scaffold. This polypeptide was shown to be chicken topoisomerase II. In the experiments described in the present article we use indirect immunofluorescence and immunoelectron microscopy to examine the distribution of topoisomerase II within intact chromosomes. We also describe a simple experimental protocol that differentiates antigens that are interspersed along the chromatin fiber from those that occupy restricted domains within the chromosome. These experiments indicate that the distribution of the enzyme appears to be independent of the bulk chromatin. Our data suggest that topoisomerase II is bound to the bases of the radial loop domains of mitotic chromosomes.