Expression of genes of potential importance in the response to chemotherapy and DNA repair in patients with ovarian cancer
Expression of genes of potential importance in the response to chemotherapy and DNA repair in patients with ovarian cancer
复制标题
DOI：
10.1006/gyno.1996.4609
复制
发表时间：
1997-04-01
影响因子：
4.7
通讯作者：
DIncalci, M
中科院分区：
文献类型：
--
作者：
Codegoni, AM;Broggini, M;DIncalci, M
在33例未经治疗的卵巢癌患者中评估了可能参与DNA修复和细胞对化疗反应的不同基因的表达。在相同患者的活检组织中，通过北方印迹分析评估修复基因O-6-甲基鸟嘌呤DNA甲基转移酶（MGMT）、3-甲基腺嘌呤DNA糖基化酶（MAG）、ERCC 1、MDR-1、DNA拓扑异构酶I、DNA拓扑异构酶II α和谷胱甘肽S-转移酶-π（GST-pi）的表达。在这些基因的表达和对化疗（主要是铂类药物，有或没有阿霉素和环磷酰胺）的反应之间没有发现直接的统计学相关性。单因素分析显示ERCC 1表达与死亡率呈弱负相关（P = 0.037），而其他参数无统计学显著相关性。编码P-糖蛋白P-170的MDR-1基因在这些患者中大多数检测不到（通过北方印迹法评估），而在大多数患者中发现相对高水平的MAG和MGMT。DNA拓扑异构酶I的表达与ERCC 1（P = 0.0026）或GST-pi（P = 0.0279）的表达之间存在显著相关性。(C)北京：科学出版社.
The expression of different genes potentially involved in DNA repair and in cell responses to chemotherapy was evaluated in 33 previously untreated ovarian cancer patients. In biopsies of the same patients the expression of repair genes O-6-methylguanine DNA methyltransferase (MGMT), 3-methyladenine DNA glycosylase (MAG), ERCC1, MDR-1, DNA topoisomerase I, DNA topoisomerase II alpha, and glutathione S-transferase-pi (GST-pi) was assessed by Northern blot analysis. No direct statistical correlation was found between the expression of these genes and the response to chemotherapy (mainly platinum-based with or without doxorubicin and cyclophosphamide). Univariate analysis showed a weak negative correlation (P = 0.037) between the expression of ERCC1 and mortality, whereas no statistically significant correlation was found for other parameters. The MDR-1 gene encoding for the P-glycoprotein P-170 was mostly undetectable in these patients (as assessed by Northern blotting), whereas relatively high levels of MAG and MGMT were found in the majority of patients. A statistically significant correlation was found between the expression of DNA topoisomerase I and the expression of either ERCC1 (P = 0.0026) or GST-pi (P = 0.0279). (C) 1997 Academic Press.