Expression of extracellular matrix metalloproteinase inducer glycosylation and caveolin-1 in healthy and inflamed human gingiva.
Expression of extracellular matrix metalloproteinase inducer glycosylation and caveolin-1 in healthy and inflamed human gingiva.
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DOI：
10.1111/jre.12095
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发表时间：
2014-04
影响因子：
3.5
通讯作者：
Jiahong Wang;D. Yang;Chengzhang Li;Shuhuan Shang;J. Xiang
中科院分区：
文献类型：
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作者：
Jiahong Wang;D. Yang;Chengzhang Li;Shuhuan Shang;J. Xiang
背景与目的糖基化细胞外基质金属蛋白酶诱导因子（EMMPRIN）与小窝蛋白-1特异性结合，影响小窝蛋白-1诱导基质金属蛋白酶（MMPs）产生的能力。本研究调查了EMMPRIN糖基化和小窝蛋白-1在健康和发炎的人牙龈组织中的表达，分析了EMMPRIN糖基化和小窝蛋白-1表达之间的关系，并评估了这种相互作用如何影响MMP-1的产生。材料与方法收集10例健康人和15例慢性牙周炎患者的牙龈组织。免疫组化检测EMMPRIN、Caveolin-1和MMP-1的表达。免疫荧光法检测EMMPRIN和小窝蛋白-1共定位。通过Western blot评估EMMPRIN糖基化、小窝蛋白-1、活性MMP-1和proMMP-1表达。结果EMMPRIN表达于牙龈上皮细胞、炎症细胞、内皮细胞和成纤维细胞样细胞。在牙龈上皮细胞和内皮细胞中检测到强烈的小窝蛋白-1免疫反应。双免疫荧光研究显示，EMMPRIN和小窝蛋白-1共定位于牙龈上皮，内皮细胞和成纤维细胞样细胞。与健康对照组相比，慢性牙周炎组高糖型EMMPRIN（HG-EMMPRIN）和活性MMP-1表达增加（p < 0.05）。HG-EMMPRIN水平与MMP-1前体及活性MMP-1蛋白水平呈正相关（p < 0.05）。结论EMMPRIN和caveolin-1在牙周组织中存在共定位。活性MMP-1和proMMP-1产生的增加可能与HG-EMMPRIN水平升高有关。
BACKGROUND AND OBJECTIVE Glycosylated extracellular matrix metalloproteinase inducer (EMMPRIN) is specifically associated with caveolin-1 and influences its ability to induce matrix metalloproteinases (MMPs) production. This study investigated EMMPRIN glycosylation and caveolin-1 expression in healthy and inflamed human gingival tissues, analyzed the relationship between EMMPRIN glycosylation and caveolin-1 expression, and assessed how this interaction influenced MMP-1 production. MATERIAL AND METHODS Gingival tissues were collected from 10 healthy subjects and 15 chronic periodontitis (chronic periodontitis) subjects. EMMPRIN, caveolin-1 and MMP-1 expressions were analyzed by immunohistochemistry. EMMPRIN and caveolin-1 co-localization was detected by immunofluorescence. EMMPRIN glycosylation, caveolin-1, active MMP-1 and proMMP-1 expression was assessed by Western blot. RESULTS EMMPRIN was expressed in gingival epithelial cells, inflammatory cells, endothelial and fibroblast-like cells. Strong caveolin-1 immunoreactivity was detected in gingival epithelial and endothelial cells. Double immunofluorescence studies revealed EMMPRIN and caveolin-1 co-localization in gingival epithelium, endothelial and fibroblast-like cells. Compared with healthy subjects, the chronic periodontitis group had increased high-glycoform EMMPRIN (HG-EMMPRIN) and active MMP-1 expression (p < 0.05). Active MMP-1 and proMMP-1 protein levels were positively correlated with HG-EMMPRIN levels (p < 0.05). CONCLUSION EMMPRIN and caveolin-1 colocalize in periodontal tissues. The increased active MMP-1 and proMMP-1 production may be associated with elevated HG-EMMPRIN levels.