Effect of cyclin G2 on proliferative ability of prostate cancer PC-3 cell
Effect of cyclin G2 on proliferative ability of prostate cancer PC-3 cell
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DOI：
10.1007/s13277-013-1389-4
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发表时间：
2014-04-01
期刊：
影响因子：
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通讯作者：
Sun, G. G.
中科院分区：
文献类型：
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作者：
Cui, D. W.;Cheng, Y. J.;Sun, G. G.
本研究旨在分析细胞周期蛋白G2(Cyclin G2，CCNG2)在前列腺癌中的表达、临床意义，以及CCNG2过表达对前列腺癌细胞系的生物学效应。应用免疫组织化学和Western印迹方法检测85例前列腺癌组织和正常组织中CCNG2蛋白的表达，探讨CCNG2蛋白表达与前列腺癌临床因素的关系。将CCNG2慢病毒载体和空载体分别导入前列腺癌PC-3细胞。逆转录-聚合酶链式反应(RT-PCR)和Western印迹法检测CCNG2的mRNA水平和蛋白表达。通过四甲基偶氮唑盐比色法和细胞周期分析，探讨CCNG2表达上调对PC-3细胞生物学效应的影响。前列腺癌组织中CCNG2蛋白表达水平明显低于正常组织(P<；0.05)。CCNG2蛋白表达水平与年龄、PSA分期、肿瘤大小无关(P<；0.05)，与淋巴结转移、临床分期、Gleason评分相关(P<；0.05)。生物学功能检测结果显示，与未转染组比较，转染组PC-3细胞存活率降低，G0/G1期比例增加，CDK2蛋白表达降低(P<0.05)。CCNG2在前列腺癌中的表达降低，并与淋巴结转移、临床分期、Gleason评分显著相关，提示CCNG2可能作为前列腺癌细胞的负调控因子发挥重要作用。
This study aimed to analyze the expression, clinical significance of cyclin G2 (CCNG2) in prostate carcinoma, and the biological effect in its cell line by CCNG2 overexpression. Immunohistochemistry and Western blot were used to analyze CCNG2 protein expression in 85 cases of prostate cancer and normal tissues to study the relationship between CCNG2 expression and clinical factors. CCNG2 lentiviral vector and empty vector were, respectively, transfected into prostate cancer PC-3 cell line. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were used to detect the mRNA level and protein of CCNG2. MTT assay and cell cycle were also conducted as to the influence of the upregulated expression of CCNG2 that might be found on PC-3 cells biological effect. The level of CCNG2 protein expression was found to be significantly lower in prostate cancer tissue than normal tissues (P < 0.05). The level of CCNG2 protein expression was not correlated with age, PSA contention, and tumor size (P < 0.05), but it was correlated with lymph node metastasis, clinic stage, and Gleason score (P < 0.05). The result of biological function shown that PC-3 cell transfected CCNG2 had a lower survival fraction, more percentage of the G0/G1 phases, and lower CDK2 protein expression compared with PC-3 cell untransfected CCNG2 (P < 0.05). CCNG2 expression decreased in prostate cancer and correlated significantly with lymph node metastasis, clinic stage, and Gleason score, suggesting that CCNG2 may play important roles as a negative regulator to prostate cancer cell.