Novel immobilization method of enzymes using a hydrophilic polymer support

Novel immobilization method of enzymes using a hydrophilic polymer support
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DOI:
10.1039/b609335c
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发表时间:
2006-10-28
影响因子:
4.9
通讯作者:
Kobayashi, Shu
Kobayashi, Shu
中科院分区:
化学2区
文献类型:
--
作者:
Kobayashi, Juta;Mori, Yuichiro;Kobayashi, Shu

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利用“聚合物包埋(PI)法”在有机溶剂中用亲水性聚合物载体固定化了一种新的酶,并将其用于固定化金属催化剂;发现使用固定化脂肪酶(Calb)比使用游离脂肪酶(Calb)更能顺利地动力学拆分仲醇。
A novel immobilization of an enzyme with a hydrophilic polymer support in organic solvents has been developed utilizing the "polymer-incarcerated (PI) method", which has been used to immobilize metal catalysts; the kinetic resolution of secondary alcohols was found to proceed more smoothly using immobilized lipases (CALB) than free lipases.