Bone marrow-derived mononuclear cell transplantation in spinal cord injury patients by lumbar puncture
Bone marrow-derived mononuclear cell transplantation in spinal cord injury patients by lumbar puncture
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DOI：
10.3233/rnn-130363
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发表时间：
2014-01-01
影响因子：
2.8
通讯作者：
Ide, Chizuka
中科院分区：
文献类型：
--
作者：
Suzuki, Yoshihisa;Ishikawa, Namiko;Ide, Chizuka
目的：本研究旨在评估自体骨髓单个核细胞鞘内移植治疗脊髓损伤患者的安全性和可行性。从脊髓损伤患者的双侧髂骨获得约120 ml骨髓穿刺液。使用Ficoll密度梯度离心进行单核细胞的分离。经腰穿将骨髓单个核细胞移植到脑脊液中。在细胞移植前和细胞移植后6个月进行功能测试。结果：5例细胞移植前为AIS A，1例细胞移植后6个月转为AIS B。5例AIS B级患者中，1例转为AIS D级，2例转为AIS C级。MRI未显示任何并发症。2例患者在抽取骨髓细胞后出现轻度贫血，数周后贫血恢复正常。结论：本研究结果提示，该方法是安全可行的。
Purpose: This study was conducted to assess the safety and feasibility of intrathecal transplantation of autologous bone marrow-derived mononuclear cells for the treatment of patients with spinal cord injury.Methods: Ten patients were included in the study. Approximately 120 ml of bone marrow aspirate was obtained from bilateral iliac bone of patients with spinal cord injury. Isolation of mononuclear cells was performed using Ficoll density-gradient centrifugation. Bone marrow mononuclear cells were transplanted into cerebrospinal fluid by lumbar puncture. Functional tests were performed prior to the cell transplantation and six months after cell transplantation. The patients were carefully observed for up to six months.Results: In 5 patients with AIS A prior to cell transplantation, 1 patient converted to AIS B six months after cell transplantation. In 5 patients with AIS B, 1 patient converted to AIS D and 2 patients to AIS C. MRI did not show any complication. Two patients showed slight anemia after aspiration of bone-marrow cells, which returned to normal level within a several weeks.Conclusion: The results of this study suggest that this method may be safe and feasible.