Induction of differentiation of the human histiocytic lymphoma cell line U-937 by retinoic acid and cyclic adenosine 3':5'-monophosphate-inducing agents.
Induction of differentiation of the human histiocytic lymphoma cell line U-937 by retinoic acid and cyclic adenosine 3':5'-monophosphate-inducing agents.
复制标题
视黄酸和环腺苷 3:5-单磷酸诱导剂诱导人组织细胞淋巴瘤细胞系 U-937 的分化。
作者：
I. Olsson;T. Breitman
用0.1 ~ 1.0 μ m维甲酸（RA）培养单核细胞样人组织细胞淋巴瘤细胞系U-937，诱导其向单核细胞样细胞分化。这些诱导细胞具有吞噬性，减少硝基蓝四氮唑，并表现出增加的单磷酸己糖分流活性，与单核细胞样细胞一致。前列腺素E、霍乱毒素或二丁基环腺苷3′：5′-单磷酸均无活性，但均显著增加ra诱导的U-937分化程度。这些数据表明细胞内环腺苷3′：5′-单磷酸水平对ra诱导效应的表达具有重要意义。对RA的反应性似乎仅限于那些在成熟相对较晚阶段被阻断的白血病骨髓细胞，如早幼粒细胞HL-60和单核细胞样U-937细胞系。
The monoblast-like human histiocytic lymphoma cell line, U-937, is induced to differentiate into monocyte-like cells by incubation with 0.1 to 1.0 microM retinoic acid (RA). These induced cells are phagocytic, reduce nitroblue tetrazolium, and show an increased hexose monophosphate shunt activity, consistent with monocyte-like cells. Prostaglandin E, cholera toxin, or dibutyryl cyclic adenosine 3':5'-monophosphate, all inactive alone, increased markedly the extent of RA-induced differentiation of U-937. These data suggest that the intracellular level of cyclic adenosine 3':5'-monophosphate is of importance for expression of the RA-induced effect. Responsiveness to RA seems to be limited to those leukemic myeloid cells blocked at a relatively late stage of maturation, like the promyelocytic HL-60 and the monoblast-like U-937 cell lines.