High plasma concentrations of osteopontin in patients with interstitial pneumonia
High plasma concentrations of osteopontin in patients with interstitial pneumonia
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DOI：
10.1016/j.rmed.2004.04.018
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发表时间：
2005-01-01
影响因子：
4.3
通讯作者：
Nasu, M
中科院分区：
文献类型：
--
作者：
Kadota, J;Mizunoe, S;Nasu, M
肺泡巨噬细胞产生的骨桥蛋白（OPN）在博莱霉素（BLM）诱导的小鼠肺纤维化的发展中作为纤维化细胞因子发挥作用，并且OPN mRNA在特发性肺纤维化（IPF）患者的肺组织上表达。本研究通过分析血浆OPN浓度与肺功能之间的相关性，探讨人间质性肺炎（IP）血浆OPN水平及其与疾病严重程度的关系。采用抗原捕获酶联免疫吸附法测定了17例IP患者、9例结节病患者和20例健康对照者血浆中OPN的浓度。IP患者血浆OPN浓度显著高于结节病患者和对照组。基于受试者工作特征曲线分析，300 - 380 ng/ml的临界点区分IP和对照受试者，灵敏度和特异性均为100%。在这种情况下，结节病的敏感性降低（55.5-33.3%），临界值为100%的特异性。IP患者血浆OPN水平与动脉血氧分压（PaO 2）呈负相关，OPN主要定位于巨噬细胞和气道上皮。这些发现表明，血浆OPN水平与l：的存在相关，OPN在IP的开发中发挥重要作用（C）2004 Elsevier Ltd.保留所有权利。
Osteopontin (OPN) produced by alveolar macrophages functions as a fibrogenic cytokine in the development of bleomycin (BLM)-induced murine pulmonary fibrosis, and OPN mRNA is expressed on lung tissues from patients with idiopathic pulmonary fibrosis (IPF). The present study investigates plasma OPN levels in human interstitial pneumonia (IP) and their relationships with disease severity by analyzing the correlation between plasma OPN concentrations and pulmonary functions. The concentrations of OPN in plasma were measured in 17 patients with IP, in 9 with sarcoidosis and in 20 healthy controls using an antigen-capture enzyme-linked immunosorbent assay. The concentrations of OPN in plasma were significantly higher in IP patients than in those with sarcoidosis or in controls. Based on a Receiver Operating Characteristic curve analysis, cut-off points between 300 and 380ng/ml discriminated between IP and control subjects with 100% sensitivity and 100% specificity. In such case, the sensitivity for sarcoidosis decreased (55.5-33.3%) in cutoffs with 100% specificity. Plasma OPN levels inversely and closely correlated with arterial oxygen tension (PaO2) in patients with IP Immunohistochemically, OPN was localized predominantly in macrophages and airway epithelium. These findings suggest that plasma OPN levels were found to be associated with the presence of l:, and that OPN play an important role in the development of IP (C) 2004 Elsevier Ltd. All rights reserved.