Expression and assembly of active cyanobacterial ribulose-1,5-bisphosphate carboxylase/oxygenase in Escherichia coli containing stoichiometric amounts of large and small subunits.
Expression and assembly of active cyanobacterial ribulose-1,5-bisphosphate carboxylase/oxygenase in Escherichia coli containing stoichiometric amounts of large and small subunits.
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含有化学计量的大亚基和小亚基的活性蓝藻核酮糖-1,5-二磷酸羧化酶/加氧酶在大肠杆菌中的表达和组装。
DOI：
10.1073/pnas.82.18.6100
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发表时间：
1985
影响因子：
11.1
通讯作者：
Small,CL
中科院分区：
文献类型：
--
作者：
Tabita,FR;Small,CL
将蓝细菌Anacystis nidulans的核酮糖-1，5-二磷酸羧化酶/加氧酶的大小亚基基因亚克隆到质粒pUC 9中。诱导后，这两个基因在大肠杆菌中表达，亚基组装成一个有活性的全酶。从E.大肠杆菌的高比活性，并发现含有等摩尔量的大小亚基。十六聚体核酮糖二磷酸羧化酶/加氧酶在E.大肠杆菌中的小亚基为研究组装机制和小亚基在催化中的作用提供了基础。
The genes for the large and small subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase from the cyanobacterium Anacystis nidulans were subcloned into plasmid pUC9. After induction, both genes were expressed in Escherichia coli and the subunits were assembled into an active holoenzyme. The enzyme was purified from E. coli to high specific activity and was found to contain equimolar amounts of large and small subunits. The assembly of the hexadecameric ribulose bisphosphate carboxylase/oxygenase in E. coli should provide the basis for studies on the mechanism of assembly and the role of small subunits in catalysis.