A Novel Aldo-Keto Reductase (AKR17A1) of Anabaena sp. PCC 7120 Degrades the Rice Field Herbicide Butachlor and Confers Tolerance to Abiotic Stresses in E. coli.
A Novel Aldo-Keto Reductase (AKR17A1) of Anabaena sp. PCC 7120 Degrades the Rice Field Herbicide Butachlor and Confers Tolerance to Abiotic Stresses in E. coli.
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DOI：
10.1371/journal.pone.0137744
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Rai LC
中科院分区：
文献类型：
--
作者：
Agrawal C;Sen S;Yadav S;Rai S;Rai LC
从鱼腥藻(Anabaena sp.)中鉴定出一种新的醛酮还原酶AKR17A1。PCC7120，作为AKR超家族的第17个家族，来自于多种生物。AKR17A1具有典型AKR的许多特征，例如-(I)对热、UV-B和镉等多重胁迫具有耐受性，(Ii)对已知的AKR底物(吲哚和2-硝基苯甲醛)具有优异的活性，以及(Iii)依赖NADPH作为酶活性的辅助因子。本研究首次报道了AKR17A1最新的属性是其代谢丁草胺的能力，丁草胺是一种持久性稻田除草剂，对农业生态系统和非目标生物产生不利影响。AKR17A1催化丁草胺降解生成1，2-苯二元酸和2，6-二(1，1-二甲乙基)-4，-甲基苯酚为主要产物。
Present study deals with the identification of a novel aldo/keto reductase, AKR17A1 from Anabaena sp. PCC7120 and adds on as 17th family of AKR superfamily drawn from a wide variety of organisms. AKR17A1 shares many characteristics of a typical AKR such as— (i) conferring tolerance to multiple stresses like heat, UV-B, and cadmium, (ii) excellent activity towards known AKR substrates (isatin and 2-nitrobenzaldehyde), and (iii) obligate dependence on NADPH as a cofactor for enzyme activity. The most novel attribute of AKR17A1, first reported in this study, is its capability to metabolize butachlor, a persistent rice field herbicide that adversely affects agro-ecosystem and non-target organisms. The AKR17A1 catalyzed- degradation of butachlor resulted into formation of 1,2-benzene dicarboxylic acid and 2,6 bis (1,1, dimethylethyl) 4,-methyl phenol as the major products confirmed by GC-MS analysis.