Heat shock response of spirochetes.
Heat shock response of spirochetes.
复制标题
螺旋体的热休克反应。
DOI：
10.1128/iai.59.4.1572-1575.1991
复制
发表时间：
1991
影响因子：
3.1
通讯作者：
Moomaw,CR
中科院分区：
文献类型：
--
作者：
Stamm,LV;Gherardini,FC;Parrish,EA;Moomaw,CR
我们研究了热休克反应的致病螺旋体梅毒螺旋体，伯氏疏螺旋体，钩端螺旋体和某些嗜血杆菌螺旋体。转换到更高温度后合成的细胞蛋白质被[35S]蛋氨酸标记，并通过凝胶电泳和荧光分析。只有T。苍白球没有表现出明显的热休克反应。GroEL和DnaK同源物在各种物种中被鉴定，尽管这些蛋白在T.梅毒螺旋体或齿垢密螺旋体。DNA杂交研究表明，螺旋体的groEL和dnaK基因是高度保守的。
We examined the heat shock response of the pathogenic spirochetes Treponema pallidum, Borrelia burgdorferi, and Leptospira interrogans and certain saprophytic spirochetes. Cellular proteins synthesized after shifts to higher temperatures were [35S]methionine labeled and analyzed by gel electrophoresis and fluorography. Only T. pallidum failed to exhibit an obvious heat shock response. GroEL and DnaK homologs were identified in the various species, although these proteins were not thermoinducible in T. pallidum or Treponema denticola. DNA hybridization studies indicate that spirochetal groEL and dnaK genes are highly conserved.