THE EFFERENT VESTIBULAR SYSTEM IN THE CAT - A HORSERADISH-PEROXIDASE AND FLUORESCENT RETROGRADE TRACERS STUDY
THE EFFERENT VESTIBULAR SYSTEM IN THE CAT - A HORSERADISH-PEROXIDASE AND FLUORESCENT RETROGRADE TRACERS STUDY
复制标题
DOI：
10.1016/0306-4522(84)90200-8
复制
发表时间：
1984-01-01
期刊：
影响因子：
3.3
通讯作者：
SANS, A
中科院分区：
文献类型：
--
作者：
DECHESNE, C;RAYMOND, J;SANS, A
用辣根过氧化物酶(HRP)逆行轴突运输和荧光逆行双标记技术对猫前庭传出系统神经元进行了研究。传出神经元的数量明显高于之前报道的数量。对这些神经元位置的三维重建表明，它们构成了一个单一的组，并没有提供基于神经元亚群的最终特化的证据。对HRP标记的传出神经元横截面积的研究发现，同侧的群体比对侧的群体含有更多数量的小神经元。用4，6-二氨基-2-苯基吲哚-2-盐酸盐与HRP联合或坚蓝与核黄联合进行双重标记，结果表明20%的传出神经元投射到两个迷路。如此高的比例提出了这样一个问题，即这些双投射细胞的作用以及它们在前庭感受器上分支的特异性。这项研究扩展了猫以前的工作，表明存在的传出神经元的数量比迄今假设的要多得多，其中20%既有交叉投影，也有非交叉投影。
Neurons of the efferent vestibular system were investigated in the cat using retrograde axonal transport of horseradish peroxidase [HRP] and fluorescent retrograde double labeling techniques. The number of efferent neurons was clearly higher than previously reported. A 3 dimensional reconstruction of the location of these neurons showed that they constitute a single group and did not give evidence of an eventual specialization based on neuron subpopulations. A study of cross-sectional areas of the HRP-labeled efferent neurons detected that the ipsilateral population contained a larger number of small neurons than the contralateral one. Double labeling by means of either 4,6-diamidino-2-phenylindol 2HCl in combination with HRP or Fast Blue in combination with Nuclear Yellow showed that 20% of efferent neurons project to both labyrinths. Such a high percentage raises the question of the role of these double-projecting cells and the specificity of their branching on vestibular receptors. This study expands previous work in the cat demonstrating that a much greater number of efferent neurons exists than had hitherto been assumed, among them 20% have both crossed and uncrossed projections.