A study of the heterogeneity of the mucoepidermoid tumor and the implication for future therapies.
A study of the heterogeneity of the mucoepidermoid tumor and the implication for future therapies.
复制标题
粘液表皮样肿瘤异质性的研究及其对未来治疗的影响。
DOI：
10.1001/archotol.1992.01880110040010
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Barsky,SH
中科院分区：
文献类型：
--
作者：
Ross,DA;Huaman,JA;Barsky,SH
粘液表皮样癌是头颈部常见的肿瘤，其广泛分布包括大唾液腺，尤其是腮腺，口咽内的小唾液腺，喉内的部位和粘膜下支气管腺。这些肿瘤是由中间、鳞状和粘液细胞亚群组成的异质性肿瘤，传统上根据其组织学性质和侵袭和转移趋势分为低度或高度。为了更好地了解这些肿瘤的异质性，我们对从不同部位的原发性人粘液表皮样癌中获得的不同细胞亚群以及在单层培养中生长的衍生细胞系和裸鼠异种移植物进行了深入研究。流式细胞术和克隆稀释法研究表明，中间细胞作为一种储备细胞，能够分化为鳞状细胞或粘液细胞。溴脱氧尿苷摄取和Ki-67抗原表达的免疫细胞化学研究表明，而鳞状和粘液细胞是终末分化和永久性停滞在G 0，中间细胞积极进入G1和S。利用人细胞膜的侵袭研究表明，只有中间细胞能够主动侵袭。由于粘液和鳞状细胞不能进一步分裂或侵袭，因此旨在使中间细胞在体内分化为粘液或鳞状方向的治疗策略将通过降低复发率和转移发生率而作为手术的有用辅助手段。
Mucoepidermoid carcinomas are common head and neck neoplasms whose wide distribution includes the major salivary glands, especially parotid, the minor salivary glands within the oropharynx, sites within the larynx, and the submucosal bronchial glands. These tumors are heterogeneous neoplasms composed of intermediate, squamous, and mucous cell subpopulations and have been traditionally classified as low or high grade based on their histological nature and tendency for invasion and metastasis. To gain a better understanding of the heterogeneity of these tumors, we conducted in-depth studies of the different cellular subpopulations obtained from primary human mucoepidermoid carcinomas from various sites, and a derived cell line grown in monolayer culture and as a nude mouse xenograft. Flow cytometric and clonal dilution studies indicate that the intermediate cell behaves as a reserve cell capable of differentiating into either the squamous cell or mucous cell. Immunocytochemical studies of bromodeoxyuridine uptake and Ki-67 antigen expression indicate that whereas the squamous and mucous cells are terminally differentiated and permanently arrested in G0, the intermediate cell actively enters G1 and S. Studies of invasion utilizing a human amnionic membrane indicate that only the intermediate cell is capable of active invasion. Because the mucous and squamous cells are incapable of further division or invasion, therapeutic strategies aimed at differentiating the intermediate cell in vivo into either mucous or squamous directions would serve as useful adjuncts to surgery by reducing the rate of recurrence and the incidence of metastasis.