Increased expression of EZH2, a polycomb group protein, in bladder carcinoma
Increased expression of EZH2, a polycomb group protein, in bladder carcinoma
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DOI：
10.1159/000087804
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发表时间：
2005-01-01
影响因子：
1.6
通讯作者：
Ergenekon, E
中科院分区：
文献类型：
--
作者：
Arisan, S;Buyuktuncer, ED;Ergenekon, E
最近的实验表明，polycomb组基因增强子zeste同源物2（EZH 2）在许多癌症类型中高度表达。因此，我们的目的是证明EZH 2基因在膀胱移行细胞癌中的表达。患者和方法：采用逆转录酶-聚合酶链反应（RT-PCR）检测健康人膀胱标本和膀胱癌标本中EZH 2 mRNA的水平。此外，通过Western印迹和免疫组织化学测定特定蛋白质的表达以确认RT-PCR结果。结果：EZH 2在晚期膀胱癌组织中的mRNA和蛋白水平表达逐渐增强。相反，100%的对照受试者EZH 2表达阴性。EZH 2的表达在G3（92%）中比G1- G2（62 - 63%）更频繁，并且在T1- 2（72 - 85%）中比Ta（56%）更频繁。Western blot分析结果证实了RT-PCR的结果.结论：EZH 2过表达预示着膀胱癌的高频率增殖和渐进性进展。这些观察结果表明，EZH 2的表达失调与膀胱癌相关。版权所有（c）2005 S. Karger AG，巴塞尔。
Introduction: Recent experiments have demonstrated that polycomb group gene enhancer zeste homolog 2 ( EZH2) is highly expressed in many cancer types. Therefore, we aim to demonstrate EZH2 gene expression in transitional cell bladder cancer. Patients and Methods: The reverse transcriptase- polymerase chain reaction ( RT- PCR) was used for detection of EZH2 mRNA levels in healthy and cancerous human bladder specimens. Also, expression of the particular protein was determined by Western blotting and immunohistochemistry to confirm RT-PCR results. Results: Gradually increased expression of EZH2 was detected by mRNA and protein levels in highly advanced bladder cancer specimens. In contrast, 100% of control subjects were negative for EZH2 expression. The expression of EZH2 was more frequent in G3 ( 92%) than G1- G2 ( 62 - 63%) and more frequent in T1- 2 ( 72 - 85%) than Ta ( 56%). Western blot analysis results confirm the RT- PCR results. Conclusions: EZH2 overexpression precedes high frequencies of proliferation and the gradual advance of bladder cancer. These observations suggest that deregulated expression of EZH2 is associated with bladder carcinoma. Copyright (c) 2005 S. Karger AG, Basel.