The Antarctic mite, Alaskozetes antarcticus, shares bacterial microbiome community membership but not abundance between adults and tritonymphs
The Antarctic mite, Alaskozetes antarcticus, shares bacterial microbiome community membership but not abundance between adults and tritonymphs
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DOI：
10.1007/s00300-019-02582-5
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发表时间：
2019-11-01
期刊：
影响因子：
1.7
通讯作者：
Benoit, Joshua B.
中科院分区：
文献类型：
--
作者：
Holmes, Christopher J.;Jennings, Emily C.;Benoit, Joshua B.
南极螨（Alaskozetes antarcticus）广泛分布于亚南极岛屿和整个南极半岛，是该地区最丰富的陆生节肢动物之一。尽管南极南极菌在恶劣的南极条件下繁衍生息的能力令人印象深刻，但人们对该物种的生物学知之甚少。在这项研究中，我们进行了 16S rRNA 基因测序，以检查最终未成熟龄（三若虫）以及雄性和雌性成虫的微生物组。微生物组包括数量有限的微生物类和属，不同阶段之间的群落成员几乎没有差异。然而，组成微生物群落的类群丰度在成虫和三若虫之间有所不同。放线菌、黄杆菌、鞘氨醇杆菌、γ-变形菌和 Betaproteobacteria 这 5 个纲，占微生物组成的 82.0%，而五个（已确定的）属：皮肤球菌属、足细菌属、金黄杆菌属、假单胞菌属和黄杆菌属，占微生物组成的 82.0%。 占总成分的68.0%。所有调查的南极菌中存在的核心微生物组主要由黄杆菌科、丛毛单胞菌科、鞘氨醇杆菌科、几丁质噬菌科和噬细胞菌科组成，但大多数核心由低丰度的操作分类单位组成。这项综合分析揭示了不同阶段的个体之间存在多样化的微生物组，其重叠可能是由于它们作为食草动物和食腐动物的共同栖息地和共同的摄食偏好。南极螨虫的微生物组显示出比低纬度螨虫物种中观察到的更多的多样性。
The Antarctic mite (Alaskozetes antarcticus) is widely distributed on sub-Antarctic islands and throughout the Antarctic Peninsula, making it one of the most abundant terrestrial arthropods in the region. Despite the impressive ability of A. antarcticus to thrive in harsh Antarctic conditions, little is known about the biology of this species. In this study, we performed 16S rRNA gene sequencing to examine the microbiome of the final immature instar (tritonymph) and both male and female adults. The microbiome included a limited number of microbial classes and genera, with few differences in community membership noted among the different stages. However, the abundances of taxa that composed the microbial community differed between adults and tritonymphs. Five classes-Actinobacteria, Flavobacteriia, Sphingobacteriia, Gammaproteobacteria, and Betaproteobacteria-comprised similar to 82.0% of the microbial composition, and five (identified) genera-Dermacoccus, Pedobacter, Chryseobacterium, Pseudomonas, and Flavobacterium-accounted for similar to 68.0% of the total composition. The core microbiome present in all surveyed A. antarcticus was dominated by the families Flavobacteriaceae, Comamonadaceae, Sphingobacteriaceae, Chitinophagaceae and Cytophagaceae, but the majority of the core consisted of operational taxonomic units of low abundance. This comprehensive analysis reveals a diverse microbiome among individuals of different stages, with overlap likely due to their shared habitat and common feeding preferences as herbivores and detritivores. The microbiome of the Antarctic mite shows considerably more diversity than observed in mite species from lower latitudes.