ULTRASTRUCTURAL EFFECTS OF GUILLAIN-BARRE SERUM IN CULTURES CONTAINING ONLY RAT SCHWANN-CELLS AND DORSAL-ROOT GANGLION NEURONS
ULTRASTRUCTURAL EFFECTS OF GUILLAIN-BARRE SERUM IN CULTURES CONTAINING ONLY RAT SCHWANN-CELLS AND DORSAL-ROOT GANGLION NEURONS
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DOI：
10.1016/0006-8993(87)91288-1
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发表时间：
1987-09-22
期刊：
影响因子：
2.9
通讯作者：
WESSELS, MM
中科院分区：
文献类型：
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作者：
BIRCHEM, R;MITHEN, FA;WESSELS, MM
将急性格林-巴利综合征患者的血清应用于仅含有大鼠背根神经节神经元和雪旺细胞的培养物中。在临床发病的前1-3周期间，从10名患者中的4名患者采集的血清先前已显示在该培养系统中在光学显微镜水平下观察时具有显著的脱髓鞘活性。在超微结构水平进行的更详细的评估显示：（1）广泛的髓鞘相关的雪旺细胞溶解与囊泡髓鞘分解一致;（2）非髓鞘相关的雪旺细胞贪婪地吞噬坏死细胞碎片和致密髓鞘碎片;（3）神经突和非髓鞘相关的雪旺细胞保持结构未受损。用急性期血清具有细胞溶解活性的患者的恢复期血清处理的培养物对神经突或雪旺细胞均未显示出显著的超微结构损伤。这是第一个提供直接证据的电子显微镜研究，表明急性格林-巴利血清可以在不含白细胞、淋巴细胞和单核吞噬细胞的实验环境中对髓鞘相关的雪旺细胞和外周髓鞘具有细胞溶解性。
Serum from patients with the acute form of the Guillain-Barrésyndrome was applied to cultures containing only rat dorsal root ganglion neurons and Schwann cells. Serum taken from 4 of 10 patients during the first 1–3 weeks of clinical onset had previously been shown to have significant demyelinating activity in this culture system when observed at the light microscopic level. More detailed assessment made at the ultrastructural level showed that: (1) wide-spread myelin-related Schwann cell lysis occurred in concert with vesicular myelin breakdown; (2) non-myelin-related Schwann cells avidly phagocytized necrotic cell debris and fragments of compact myelin; and (3) neurites and non-myelin-related Schwann cells remained structurally undamaged. Cultures treated with convalescent phase serum from patients whose acute phase serum had cytolytic activity displayed no significant ultrastructural damage to either neurites or Schwann cells. This is the first electron microscopic study to provide direct evidence that acute Guillain-Barréserum can be cytolytic for myelin-related Schwann cells and peripheral myelin in an experimental setting free of leukocytes, lymphocytes and mononuclear phagocytes.