Cryopreservation of Canine Embryos
Cryopreservation of Canine Embryos
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DOI：
10.1095/biolreprod.110.087312
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发表时间：
2011-02-01
影响因子：
3.6
通讯作者：
Suzuki, Hiroshi
中科院分区：
文献类型：
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作者：
Abe, Yasuyuki;Suwa, Yoshinori;Suzuki, Hiroshi
辅助生殖技术（ARTs），如体外受精、胚胎移植和配子冷冻保存，除了改善人类不孕症治疗和家畜育种外，还为生物医学科学的发展做出了重大贡献。然而，与包括人类在内的其他哺乳动物相比，用于犬类的抗逆转录病毒治疗的效用有限。尽管有体细胞克隆成功的报道，但迄今为止，冷冻精液人工授精只能用于伴侣犬和工作犬以及盲人导盲犬的改良育种和繁殖。我们在这里描述了成功的胚胎冷冻保存和随后的胚胎移植在狗。人工授精后，从切除的生殖器官中收集犬胚胎，然后用玻璃化法冷冻保存。通过膀胱镜将冷冻保存的4细胞到桑葚胚非手术移植到9只受体母狗的子宫中，5只受体母狗怀孕，其中4只共产下7只幼崽。犬类胚胎的低温保存将有助于遗传物质的运输和储存，并有助于消除犬类的垂直传播疾病。此外，这项技术将有助于改善伴侣犬和工作犬的繁殖，如导盲犬、毒品探测犬和检疫犬。
The assisted reproductive techniques (ARTs) such as in vitro fertilization, embryo transfer, and cryopreservation of gametes have contributed considerably to the development of biomedical sciences in addition to improving infertility treatments in humans as well as the breeding of domestic animals. However, ARTs used in canine species have strictly limited utility when compared with other mammalian species, including humans. Although successful somatic cell cloning has been reported, artificial insemination by frozen semen to date is only available for the improved breeding and reproduction for companion and working dogs as well as guide dogs for the blind. We describe here the successful cryopreservation of embryos and subsequent embryo transfer in dogs. Canine embryos were collected from excised reproductive organs after artificial insemination and subsequently cryopreserved by a vitrification method. When the 4-cell to morula stage of cryopreserved embryos were nonsurgically transferred into the uteri of nine recipient bitches using a cystoscope, five recipients became pregnant and four of them delivered a total of seven pups. The cryopreservation of embryos in canine species will facilitate the transportation and storage of genetic materials and will aid in the elimination of vertically transmitted diseases in dogs. In addition, this technique will contribute to the improved breeding of companion and working dogs such as guide dogs, drug-detecting dogs, and quarantine dogs.